Overview

  • Product name
  • Description
    Rabbit polyclonal to ABHD6
  • Specificity
    ab74681 detects endogenous levels of total ABHD6 protein.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal sequence of human ABHD6.

  • Positive control
    • COLO cell extracts and A549 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab74681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • Relevance
    ABHD6 belongs to the alpha/beta hydrolase superfamily. It is highly expressed in mammalian brain and catalyzes the hydrolysis of the endocannabinoid 2-arachidonoyl glycerol (2-AG).
  • Cellular localization
    Membrane; Single-pass type II membrane protein
  • Database links
  • Alternative names
    • 0610041D24Rik antibody
    • 2 arachidonoylglycerol hydrolase antibody
    • AA673485 antibody
    • Abhydrolase domain containing 6 antibody
    • Abhydrolase domain containing protein 6 antibody
    • AV065425 antibody
    • Llipase protein antibody
    • MGC94917 antibody
    see all

Images

  • ab74681 at 1/500-1/1000 dilution staining ABHD6 in A549 cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
  • All lanes : Anti-ABHD6 antibody (ab74681) at 1/500 dilution

    Lane 1 : COLO cell extracts
    Lane 2 : COLO cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 39 kDa
    Observed band size : 39 kDa

References

ab74681 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cultured Cells (TSA cells)
Permeabilization
Yes - Triton
Specification
TSA cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 03 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain)
Specification
Brain
Username

Abcam user community

Verified customer

Submitted Aug 03 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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