Overview

  • Product name
  • Description
    Rabbit polyclonal to ABHD7
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: FPHPNVFTEY ILRHPAQLLK SSYYYFFQIP WFPEFMFSIN DFKVLKHLFT SHSTGIGRKG CQLTTEDLEA YIYVF, corresponding to amino acids 194-268 of Human ABHD7 (Q8IUS5).

  • Positive control
    • Human gall bladder tissue; RT-4 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab122246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABHD7 antibody
    • Abhydrolase domain containing 7 antibody
    • Abhydrolase domain-containing protein 7 antibody
    • Ephx4 antibody
    • EPHX4_HUMAN antibody
    • EPHXRP antibody
    • Epoxide hydrolase 4 antibody
    • Epoxide hydrolase-related protein antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab122246 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-ABHD7 antibody (ab122246) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Liver lysate
    Lane 5 : Tonsil lysate
  • ab122246, at a 1/150 dilution, staining ABHD7 in paraffin embedded Human Gall bladder tissue by Immunohistochemistry.

References

ab122246 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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