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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/250 - 1/500.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-ABHD7 antibody images
Immunocytochemistry/ Immunofluorescence - Anti-ABHD7 antibody (ab122246)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in vesicles. Recommended concentration of ab122246 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-ABHD7 antibody (ab122246)
All lanes : Anti-ABHD7 antibody (ab122246) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Liver lysate
Lane 5 : Tonsil lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABHD7 antibody (ab122246)
ab122246, at a 1/150 dilution, staining ABHD7 in paraffin embedded Human Gall bladder tissue by Immunohistochemistry.
References for Anti-ABHD7 antibody (ab122246)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"