Overview

  • Product nameAnti-ABI2 antibody
    See all ABI2 primary antibodies
  • Description
    Rabbit polyclonal to ABI2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla, Opossum, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within internal sequence amino acids 351-401 of Human ABI2 (NP_005750.4).

  • Positive control
    • Whole cell lysate from HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab113991 was affinity purified using an epitope specific to ABI2 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab113991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 56 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionMay act in regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA.
  • Sequence similaritiesBelongs to the ABI family.
    Contains 1 SH3 domain.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Post-translational
    modifications
    Is a substrate for ABL1.
  • Cellular localizationCytoplasm and Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abelson interactor 2 antibody
    • ABI 2 antibody
    • Abi-2 antibody
    • ABI2 antibody
    • ABI2_HUMAN antibody
    • ABI2B antibody
    • Abl binding protein 3 antibody
    • Abl interacting protein 1 (SH3 containing protein) antibody
    • Abl interactor 2 antibody
    • Abl interactor protein 2b antibody
    • Abl-binding protein 3 antibody
    • AblBP3 antibody
    • AIP 1 antibody
    • Arg binding protein 1 antibody
    • Arg protein tyrosine kinase binding protein antibody
    • Arg-binding protein 1 antibody
    • ArgBP1 antibody
    • ARGBPIA antibody
    • ArgBPIB antibody
    • OTTHUMP00000163783 antibody
    • OTTHUMP00000163784 antibody
    • OTTHUMP00000206182 antibody
    • OTTHUMP00000206183 antibody
    • OTTHUMP00000206184 antibody
    • OTTHUMP00000206185 antibody
    • SSH3BP2 antibody
    see all

Anti-ABI2 antibody images

  • All lanes : Anti-ABI2 antibody (ab113991) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 56 kDa


    Exposure time : 30 seconds
  • ab113991, at 1 µg/ml, detecting ABI2 by WB following immunoprecipitation.
    Lane 1: IP using a rabbit anti-ABI2 which recognizes an upstream epitope
    Lane 2: IP using ab113991
    Lane 3; IP using control IgG.
    ab113991 was used for immunoprecipitation at 3 µg/mg of HeLa whole cell lysate. 1 mg of lysate was used for IP and 20% of IP was loaded.
    Detection: Chemiluminescence with a 3 minute exposure time.

References for Anti-ABI2 antibody (ab113991)

ab113991 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"