Overview

  • Product nameAnti-ABI2 antibody
    See all ABI2 primary antibodies
  • Description
    Mouse polyclonal to ABI2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    ABI2 (NP_005750.4, 475 a.a), full-length human protein.

  • Positive control
    • ABI2 transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionMay act in regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA.
  • Sequence similaritiesBelongs to the ABI family.
    Contains 1 SH3 domain.
    Contains 1 t-SNARE coiled-coil homology domain.
  • Post-translational
    modifications
    Is a substrate for ABL1.
  • Cellular localizationCytoplasm and Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips.
  • Information by UniProt
  • Database links
  • Alternative names
    • Abelson interactor 2 antibody
    • ABI 2 antibody
    • Abi-2 antibody
    • ABI2 antibody
    • ABI2_HUMAN antibody
    • ABI2B antibody
    • Abl binding protein 3 antibody
    • Abl interacting protein 1 (SH3 containing protein) antibody
    • Abl interactor 2 antibody
    • Abl interactor protein 2b antibody
    • Abl-binding protein 3 antibody
    • AblBP3 antibody
    • AIP 1 antibody
    • Arg binding protein 1 antibody
    • Arg protein tyrosine kinase binding protein antibody
    • Arg-binding protein 1 antibody
    • ArgBP1 antibody
    • ARGBPIA antibody
    • ArgBPIB antibody
    • OTTHUMP00000163783 antibody
    • OTTHUMP00000163784 antibody
    • OTTHUMP00000206182 antibody
    • OTTHUMP00000206183 antibody
    • OTTHUMP00000206184 antibody
    • OTTHUMP00000206185 antibody
    • SSH3BP2 antibody
    see all

Anti-ABI2 antibody images

  • All lanes : Anti-ABI2 antibody (ab70144) at 1/500 dilution

    Lane 1 : transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 52 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab70144 at 10µg/ml staining ABl2 in Hela cells by Immunocytochemistry/Immunofluorescence.

References for Anti-ABI2 antibody (ab70144)

This product has been referenced in:
  • Maddala R  et al. Rac1 GTPase-deficient mouse lens exhibits defects in shape, suture formation, fiber cell migration and survival. Dev Biol : (2011). WB ; Mouse . Read more (PubMed: 21945075) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"