1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/500 - 1/1000.
Application notesIs unsuitable for Flow Cyt.
FunctionMay act in regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA.
Sequence similaritiesBelongs to the ABI family. Contains 1 SH3 domain. Contains 1 t-SNARE coiled-coil homology domain.
Post-translational modificationsIs a substrate for ABL1.
Cellular localizationCytoplasm and Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips.
Western blot analysis of Human fibroblast cell lysates (15μg/lane) labelling ABI2 with ab108340 at 1:1000. A Dyomics® 647-conjugated Goat anti-rabbit IgG monoclonal (1:5000) was used as the secondary antibody.
ab108340, at 1/500, staining ABI2 in HeLa cells by Immunofluorescence.
References for Anti-ABI2 antibody [EPR3906] (ab108340)
This product has been referenced in:
Crawford LJ et al. Synergistic effects of proteasome inhibitor carfilzomib in combination with tyrosine kinase inhibitors in imatinib-sensitive and -resistant chronic myeloid leukemia models. Oncogenesis3:e90 (2014).
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