Anti-ABI2 antibody [EPR3906] (ab108340)
- Datasheet
- Specific References (1)
- Protocols
Overview
- Product nameAnti-ABI2 antibody [EPR3906]
See all ABI2 primary antibodies - DescriptionRabbit monoclonal [EPR3906] to ABI2
- Tested applicationsSuitable for:
WB, IP, IHC-P, ICCmore details
Unsuitable for: Flow Cyt - Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide corresponding to a region within in Human ABI2.
- Positive control
- HeLa, Jurkat, K562, SH-SY5Y and HepG2 cell lysates. Paraffin-embedded Human brain tissue.
- General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- ClonalityMonoclonal
- Clone numberEPR3906
- IsotypeIgG
- Research areas
Associated products
- Positive Controls
Applications
Our Abpromise guarantee covers the use of ab108340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 57 kDa. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | 1/500 - 1/1000. |
Target
- FunctionMay act in regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Regulates ABL1/c-Abl-mediated phosphorylation of MENA.
- Sequence similaritiesBelongs to the ABI family.
Contains 1 SH3 domain.
Contains 1 t-SNARE coiled-coil homology domain. - Post-translational
modificationsIs a substrate for ABL1. - Cellular localizationCytoplasm and Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Isoform 1 but not isoform 3 is localized to protruding lamellipodia and filopodia tips.
- Information by UniProt
-
Database links
- Entrez Gene: 10152 Human
- Entrez Gene: 329165 Mouse
- Entrez Gene: 286928 Rat
- Omim: 606442 Human
- SwissProt: Q9NYB9 Human
- SwissProt: P62484 Mouse
- Unigene: 471156 Human
- Unigene: 212066 Mouse
see all -
Alternative names
- Abelson interactor 2 antibody
- ABI 2 antibody
- Abi-2 antibody
see all
Anti-ABI2 antibody [EPR3906] images
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![Western blot - Anti-ABI2 antibody [EPR3906] (ab108340)](http://a.abcam.com/ps/products/108/ab108340/Images/ab108340-203621-ab108340wb.jpg)
Western blot - Anti-ABI2 antibody [EPR3906] (ab108340)This image is courtesy of an anonymous Abreview
Performed under reducing conditions.
Predicted band size : 57 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
Exposure time : 2 minutesThis image is courtesy of an anonymous Abreview
Western blot analysis of Human fibroblast cell lysates (15μg/lane) labelling ABI2 with ab108340 at 1:1000. A Dyomics® 647-conjugated Goat anti-rabbit IgG monoclonal (1:5000) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABI2 antibody [EPR3906] (ab108340)](http://a.abcam.com/ps/datasheet/images/108/ab108340/ABI2-Primary-antibodies-ab108340-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ABI2 antibody [EPR3906] (ab108340)ab108340, at 1/250, staining ABI2 in paraffin-embedded Human brain tissue by Immunohistochemistry. -
![Immunocytochemistry/ Immunofluorescence - ABI2 antibody [EPR3906] (ab108340)](http://a.abcam.com/ps/datasheet/images/108/ab108340/ABI2-Primary-antibodies-ab108340-3.jpg)
Immunocytochemistry/ Immunofluorescence - ABI2 antibody [EPR3906] (ab108340)ab108340, at 1/500, staining ABI2 in HeLa cells by Immunofluorescence.
References for Anti-ABI2 antibody [EPR3906] (ab108340)
This product has been referenced in:
- Crawford LJ et al. Synergistic effects of proteasome inhibitor carfilzomib in combination with tyrosine kinase inhibitors in imatinib-sensitive and -resistant chronic myeloid leukemia models. Oncogenesis 3:e90 (2014). WB ; Human . Read more (PubMed: 24590311) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"