Overview

  • Product name
  • Description
    Rabbit polyclonal to ABLIM1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Opossum, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 50 and 100 of human ABLIM1 (NP_002304.3)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab85808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
IP Use at 5-10 µg/mg of lysate.

Target

  • Function
    May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.
  • Tissue specificity
    Detected in liver, heart, skeletal muscle, brain and retina, where it is concentrated in the inner segment and in the outer plexiform layers.
  • Sequence similarities
    Contains 1 HP (headpiece) domain.
    Contains 4 LIM zinc-binding domains.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABLIM 1 antibody
    • ABLIM antibody
    • abLIM-1 antibody
    • ABLIM-L antibody
    • ABLIM-M antibody
    • ABLIM-S antibody
    • ABLIM1 antibody
    • ABLM1_HUMAN antibody
    • Actin binding double zinc finger protein antibody
    • Actin binding LIM protein 1 antibody
    • Actin binding LIM protein family member 1 antibody
    • Actin-binding double zinc finger protein antibody
    • Actin-binding LIM protein 1 antibody
    • Actin-binding LIM protein family member 1 antibody
    • DKFZp781D0148 antibody
    • FLJ14564 antibody
    • KIAA0059 antibody
    • LIM actin binding protein 1 antibody
    • LIMAB 1 antibody
    • LIMAB1 antibody
    • Limatin antibody
    • MGC1224 antibody
    • MGC125264 antibody
    • OTTHUMP00000020530 antibody
    • OTTHUMP00000020531 antibody
    • OTTHUMP00000020532 antibody
    • OTTHUMP00000020533 antibody
    • OTTHUMP00000020534 antibody
    • OTTHUMP00000020535 antibody
    • OTTHUMP00000020536 antibody
    • OTTHUMP00000020537 antibody
    • RGD1565768 antibody
    • RP11-317F20.1 antibody
    see all

Images

  • All lanes : Anti-ABLIM1 antibody (ab85808) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 88 kDa
    Observed band size : 88 kDa


    Exposure time : 3 minutes
  • Detection of ABLIM1 by Western Blot of Immunprecipitate.
    ab85808 at 1µg/ml staining ABLIM1 in HeLa whole cell lysate immunoprecipitated using ab85808 at 10µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab85808 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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