Overview

  • Product nameAnti-ABLIM3 antibodySee all ABLIM3 primary antibodies ...
  • Description
    Mouse polyclonal to ABLIM3
  • Tested applicationsWB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-544 of Human ABLIM3

Properties

Applications

Our Abpromise guarantee covers the use of ab67721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 78 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionMay act as scaffold protein. May stimulate ABRA activity and ABRA-dependent SRF transcriptional activity.
  • Tissue specificityExpressed predominantly in heart and brain.
  • Sequence similaritiesContains 1 HP (headpiece) domain.
    Contains 4 LIM zinc-binding domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • abLIM-3 antibody
    • Ablim3 antibody
    • ABLM3_HUMAN antibody
    • Actin binding LIM protein 3 antibody
    • Actin binding LIM protein family member 3 antibody
    • Actin-binding LIM protein 3 antibody
    • Actin-binding LIM protein family member 3 antibody
    • HMFN1661 antibody
    • KIAA0843 antibody
    see all

Anti-ABLIM3 antibody images

  • All lanes : Anti-ABLIM3 antibody (ab67721) at 1/500 dilution

    Lane 1 : ABLIM3 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution

    Predicted band size : 78 kDa
  • ab67721 staining ABLIM3 in murine cerebellum tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed with acetone, blocked with 1% BSA for 1 hour at 25°C and then incubated with ab67721 at a 1/200 dilution for 24 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-mouse polyclonal used at a 1/400 dilution.

    See Abreview

References for Anti-ABLIM3 antibody (ab67721)

ab67721 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Amar Sahay

Verified customer

Submitted Mar 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"