Overview

  • Product nameAnti-ABLIM3 antibodySee all ABLIM3 primary antibodies ...
  • Description
    Mouse polyclonal to ABLIM3
  • Tested applicationsWB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-544 of Human ABLIM3

Properties

Applications

Our Abpromise guarantee covers the use of ab67721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 78 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionMay act as scaffold protein. May stimulate ABRA activity and ABRA-dependent SRF transcriptional activity.
  • Tissue specificityExpressed predominantly in heart and brain.
  • Sequence similaritiesContains 1 HP (headpiece) domain.
    Contains 4 LIM zinc-binding domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • abLIM-3 antibody
    • Ablim3 antibody
    • ABLM3_HUMAN antibody
    • Actin binding LIM protein 3 antibody
    • Actin binding LIM protein family member 3 antibody
    • Actin-binding LIM protein 3 antibody
    • Actin-binding LIM protein family member 3 antibody
    • HMFN1661 antibody
    • KIAA0843 antibody
    see all

Anti-ABLIM3 antibody images

  • All lanes : Anti-ABLIM3 antibody (ab67721) at 1/500 dilution

    Lane 1 : ABLIM3 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution

    Predicted band size : 78 kDa
  • ab67721 staining ABLIM3 in murine cerebellum tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed with acetone, blocked with 1% BSA for 1 hour at 25°C and then incubated with ab67721 at a 1/200 dilution for 24 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-mouse polyclonal used at a 1/400 dilution.

    See Abreview

References for Anti-ABLIM3 antibody (ab67721)

ab67721 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Cerebellum)
Specification Cerebellum
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Amar Sahay

Verified customer

Submitted Mar 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"