Chromogenic peroxidase substrate widely utilized in enzyme-linked immunosorbent assay (ELISA) procedures. Produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. Also used in the food industry to measure the antioxidant capacities of foods.
Store at +4°C. The product can be stored for up to 12 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Wu X et al. Electrochemical approach for detection of extracellular oxygen released from erythrocytes based on graphene film integrated with laccase and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid). Anal Chem82:3588-96 (2010).
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Szutowicz A et al. Colorimetric assay for monoamine oxidase in tissues using peroxidase and 2,2'-azinodi(3-ethylbenzthiazoline-6-sulfonic acid) as chromogen. Anal Biochem138:86-94 (1984).
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