Overview

  • Product name
    Anti-ACAA1 antibody [2H6BE7]
    See all ACAA1 primary antibodies
  • Description
    Mouse monoclonal [2H6BE7] to ACAA1
  • Tested applications
    Suitable for: ICC, Flow Cyt, IP, WB, In-Cell ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Human liver mitochondria

  • Positive control
    • HepG2,HL-60 cells, human liver tissue, rat liver tissue and mouse liver tissue.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 5 µg/ml. Heat-induced antigen retrieval improves signal
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
In-Cell ELISA Use a concentration of 1 µg/ml.

Target

Images

  • ACAA1 was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Mouse monoclonal to ACAA1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab110289.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 44kDa; ACAA1

  • ab110289, at 5 µg/mL, staining ACAA1 in Human HepG2 cells. DAPI was used to stain the cell nuclei (blue).
  • ab110289, at 1 µg/mL, staining ACAA1 in HL-60 cells (blue) or using an isotype control (red).
  • All lanes : Anti-ACAA1 antibody [2H6BE7] (ab110289) at 1 µg/ml

    Lane 1 : Human liver
    Lane 2 : HepG2
    Lane 3 : Rat liver
    Lane 4 : Mouse liver

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 44 kDa

References

This product has been referenced in:

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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