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Product name Anti-ACAA1 antibody [2H6BE7] See all ACAA1 primary antibodies
Mouse monoclonal [2H6BE7] to ACAA1
Tested applications Suitable for:
ICC, Flow Cyt, IP, WB, In-Cell ELISA more details
Mouse, Rat, Human
Human liver mitochondria
HepG2,HL-60 cells, human liver tissue, rat liver tissue and mouse liver tissue.
Product was previously marketed under the MitoSciences sub-brand.
Storage instructions Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer Preservative: 0.02% Sodium azide Constituent: HBS
Concentration information loading...
Purity Ammonium Sulphate Precipitation
Purification notes Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation.
Clone number 2H6BE7
Light chain type kappa
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Heat-induced antigen retrieval improves signal
Use a concentration of 1 µg/ml.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use a concentration of 5 µg/ml.
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
Use a concentration of 1 µg/ml.
Pathway Lipid metabolism; fatty acid metabolism.
Sequence similarities Belongs to the thiolase family.
Cellular localization Peroxisome.
Information by UniProt
3 ketoacyl CoA thiolase peroxisomal antibody
3-ketoacyl-CoA thiolase antibody
Anti-ACAA1 antibody [2H6BE7] images
Immunoprecipitation - Anti-ACAA1 antibody [2H6BE7] (ab110289)
ACAA1 was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Mouse monoclonal to ACAA1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab110289.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.
Band: 44kDa; ACAA1
Immunocytochemistry - ACAA1 antibody [2H6BE7 ] (ab110289)
ab110289, at 5 µg/mL, staining ACAA1 in Human HepG2 cells. DAPI was used to stain the cell nuclei (blue).
Flow Cytometry - ACAA1 antibody [2H6BE7 ] (ab110289)
ab110289, at 1 µg/mL, staining ACAA1 in HL-60 cells (blue) or using an isotype control (red).
Western blot - Anti-ACAA1 antibody [2H6BE7 ] (ab110289)
All lanes : Anti-ACAA1 antibody [2H6BE7] (ab110289) at 1 µg/ml Lane 1 : Human liver Lane 2 : HepG2 Lane 3 : Rat liver Lane 4 : Mouse liver Lysates/proteins at 10 µg per lane. Predicted band size : 44 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"