Anti-ACAA1/Beta-ketothiolase antibody (ab154091)
Key features and details
- Rabbit polyclonal to ACAA1/Beta-ketothiolase
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ACAA1/Beta-ketothiolase antibody
See all ACAA1/Beta-ketothiolase primary antibodies -
Description
Rabbit polyclonal to ACAA1/Beta-ketothiolase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human ACAA1/Beta-ketothiolase aa 50-350.
Database link: P09110 -
Positive control
- WB: A549, HeLa and mouse liver whole cell lysates; ICC/IF: HeLa cells; IHC-P: Mouse stomach;
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
Target
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Pathway
Lipid metabolism; fatty acid metabolism. -
Sequence similarities
Belongs to the thiolase family. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 508324 Cow
- Entrez Gene: 30 Human
- Entrez Gene: 113868 Mouse
- Omim: 604054 Human
- SwissProt: P09110 Human
- SwissProt: Q921H8 Mouse
- Unigene: 643487 Human
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Alternative names
- 3 ketoacyl CoA thiolase peroxisomal antibody
- 3-ketoacyl-CoA thiolase antibody
- ACAA antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse stomach staining ACAA1/Beta-ketothiolase with ab154091 at a dilution of 1/500.
EDTA based Antigen Retrieval, pH 8.0 for 15min.
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Immunofluorescent analysis of methanol-fixed HeLa cells labeling ACAA1/Beta-ketothiolase with ab154091 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
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All lanes : Anti-ACAA1/Beta-ketothiolase antibody (ab154091) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 44 kDa
10% SDS PAGE -
Anti-ACAA1/Beta-ketothiolase antibody (ab154091) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 44 kDa
10% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling ACAA1/Beta-ketothiolase with ab154091 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154091 has been referenced in 1 publication.
- Colasuonno F et al. Mitochondrial and Peroxisomal Alterations Contribute to Energy Dysmetabolism in Riboflavin Transporter Deficiency. Oxid Med Cell Longev 2020:6821247 (2020). PubMed: 32855765