Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (ab210961)
Key features and details
- Mouse monoclonal [CL2650] to ACAA1/Beta-ketothiolase
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ACAA1/Beta-ketothiolase antibody [CL2650]
See all ACAA1/Beta-ketothiolase primary antibodies -
Description
Mouse monoclonal [CL2650] to ACAA1/Beta-ketothiolase -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment (proprietary-tag) corresponding to Human ACAA1/Beta-ketothiolase aa 50-200.
Database link: P09110 -
Epitope
Binds to an epitope located within the peptide sequence GAIMARIAQFLSDIP as determined by overlapping synthetic peptides. -
Positive control
- IHC-P: Human colon, liver, kidney and prostate tissues. WB: U-251 Cells ICC/IF: CACO-2
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL2650 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
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ICC/IF |
Use a concentration of 2 - 10 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa. |
ICC/IF
Use a concentration of 2 - 10 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Pathway
Lipid metabolism; fatty acid metabolism. -
Sequence similarities
Belongs to the thiolase family. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 30 Human
- Entrez Gene: 113868 Mouse
- Entrez Gene: 24157 Rat
- Omim: 604054 Human
- SwissProt: P09110 Human
- SwissProt: Q921H8 Mouse
- SwissProt: P07871 Rat
- SwissProt: P21775 Rat
see all -
Alternative names
- 3 ketoacyl CoA thiolase peroxisomal antibody
- 3-ketoacyl-CoA thiolase antibody
- ACAA antibody
see all
Images
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PFA-fixed, Triton X-100 permeabilized CACO-2 cells stained for ACAA1 (Green) using ab210961 at 1 µg/ml. Microtubules are stained red, nuclei are stained blue.
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Anti-ACAA1/Beta-ketothiolase antibody [CL2650] (ab210961) at 1/500 dilution + U251 cells
Predicted band size: 44 kDa -
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human prostate tissue labeling ACAA1/Beta-ketothiolase with ab210961 at 1/200 dilution.
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Immunohistochemical analysis of formalin-fixed paraffin-embedded Human kidney tissue labeling ACAA1/Beta-ketothiolase with ab210961 at 1/200 dilution.
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Immunohistochemical analysis of formalin-fixed paraffin-embedded Human liver tissue labeling ACAA1/Beta-ketothiolase with ab210961 at 1/200 dilution.
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Immunohistochemical analysis of formalin-fixed paraffin-embedded Human colon tissue labeling ACAA1/Beta-ketothiolase with ab210961 at 1/200 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab210961 has not yet been referenced specifically in any publications.