Overview

  • Product name
  • Description
    Rabbit polyclonal to ACADL
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 288-337 (LPQERLLIAD VAISASEFMF EETRNYVKQR KAFGKTVAHL QTVQHKLAEL) of human ACADL (NP_001599).

  • Positive control
    • Placental lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab82853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Pathway
    Lipid metabolism; mitochondrial fatty acid beta-oxidation.
  • Involvement in disease
    Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD) [MIM:201475]. An inborn error of mitochondrial fatty acid beta-oxidation which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form characterized by early onset, high mortality and high incidence of cardiomyopathy; a milder childhood form with later onset, characterized by hypoketotic hypoglycemia, low mortality and rare cardiomyopathy; an adult form, with isolated skeletal muscle involvement, rhabdomyolysis and myoglobinuria, usually triggered by exercise or fasting.
  • Sequence similarities
    Belongs to the acyl-CoA dehydrogenase family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAD4 antibody
    • ACADL antibody
    • ACADL_HUMAN antibody
    • Acyl Coenzyme A dehydrogenase long chain antibody
    • Acyl-CoA dehydrogenase long chain antibody
    • FLJ94052 antibody
    • LCAD antibody
    • Long chain acyl CoA dehydrogenase antibody
    • Long-chain specific acyl-CoA dehydrogenase, mitochondrial antibody
    see all

Images

  • Anti-ACADL antibody (ab82853) at 1 µg/ml (in 5% skim milk / PBS buffer) + placental lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • LaBarge SA  et al. p300 is not required for metabolic adaptation to endurance exercise training. FASEB J 30:1623-33 (2016). Read more (PubMed: 26712218) »
  • Mells JE  et al. Glp-1 analog, liraglutide, ameliorates hepatic steatosis and cardiac hypertrophy in C57BL/6J mice fed a Western diet. Am J Physiol Gastrointest Liver Physiol 302:G225-35 (2012). WB ; Mouse . Read more (PubMed: 22038829) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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