• Product nameAnti-ACADM antibody [3B7BH7]
    See all ACADM primary antibodies
  • Description
    Mouse monoclonal [3B7BH7] to ACADM
  • Tested applicationsSuitable for: WB, IP, Flow Cyt, In-Cell ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Recombinant human MCAD

  • Positive control
    • HeLa cells, HL-60 cells, Human cerebellum, HepG2 cells, HeLa cells, H9C2 (rat cells), and MEF (mouse cells) lysates.
  • General notes

    MCAD (medium-chain acyl-CoA dehydrogenase) is an oxidoreductase enzyme of the mitochondrial fatty acid beta-oxidation pathway that is specific for acyl chain lengths of 4 to 16. It also utilizes the electron transfer flavoprotein (ETF) as electron acceptor that transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).

    Product was previously marketed under the MitoSciences sub-brand.


    Alternative versions available:

    Anti-ACADM antibody (Alexa Fluor® 488) [3B7BH7] (ab198078)

    Anti-ACADM antibody (Alexa Fluor® 647) [3B7BH7] (ab198099)



Our Abpromise guarantee covers the use of ab110296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.125 µg/ml. Predicted molecular weight: 47 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
In-Cell ELISA Use a concentration of 1 µg/ml. (0.1 µg/well)
IHC-P 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.


  • FunctionThis enzyme is specific for acyl chain lengths of 4 to 16.
  • PathwayLipid metabolism; mitochondrial fatty acid beta-oxidation.
  • Involvement in diseaseDefects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
  • Sequence similaritiesBelongs to the acyl-CoA dehydrogenase family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAD 1 antibody
    • ACAD1 antibody
    • Acadm antibody
    • ACADM_HUMAN antibody
    • Acyl coenzyme A dehydrogenase antibody
    • Acyl coenzyme A dehydrogenase C 4 to C 12 straight chain antibody
    • FLJ18227 antibody
    • FLJ93013 antibody
    • FLJ99884 antibody
    • MCAD antibody
    • MCADH antibody
    • Medium chain acyl CoA dehydrogenase antibody
    • Medium chain fatty acyl CoA dehydrogenase antibody
    • Medium chain specific acyl CoA dehydrogenase antibody
    • Medium chain specific acyl CoA dehydrogenase mitochondrial antibody
    • Medium-chain specific acyl-CoA dehydrogenase antibody
    • mitochondrial antibody
    see all

Anti-ACADM antibody [3B7BH7] images

  • Immunocytochemistry image of ab110296 stained Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110296 (1 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
  • ACADM immunohistochemistry in Human cerebellum visualized with ab110296 at 1/1000. MCAD immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells. Note the distinctive subcellular localization of MCAD immunoreactivity in the Purkinje cell bodies.
  • All lanes : Anti-ACADM antibody [3B7BH7] (ab110296) at 0.125 µg/ml

    Lane 1 : HepG2 cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : H9C2 (rat cells) lysates
    Lane 4 : MEF (mouse cells) lysates

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 47 kDa
  • HL-60 cells were stained with 1 µg/mL ab110296 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa

    HL-60 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of fenofibrate (ab120832). Increased expression of ACADM in HL-60 cells correlates with an increase in fenofibrate concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab110296 at 1 µg/ml and ab9484 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.

References for Anti-ACADM antibody [3B7BH7] (ab110296)

This product has been referenced in:
  • Cornelius N  et al. Cellular consequences of oxidative stress in riboflavin responsive multiple acyl-CoA dehydrogenation deficiency patient fibroblasts. Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 24698980) »
  • Tomilov A  et al. Shc depletion stimulates brown fat activity in vivo and in vitro. Aging Cell 13:1049-58 (2014). Mouse . Read more (PubMed: 25257068) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Heart tissue lysate)
Loading amount 30 µg
Specification Heart tissue lysate
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 30°C


Verified customer

Submitted Feb 15 2013

Vielen Dank für Ihre Antwort.

Leider ist mir bei meiner letzten Antwort ein Fehler unterlaufen und ich möchte diesen hiermit korrigieren:

ab110296 wird nicht im Kit ACADM ELISA Kit (ab129734) verwendet.. Es wird ein Antik&...

Read More

Vielen Dank für Ihre Anfrage.

Das Immunogen für ab110296 war das Protein voller Länge. Das Epitop, das der Antikörper bindet ist leider nicht näher kartiert worden und wir kennen es deshalb nicht.

Ich freue ...

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