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Recombinant human MCAD
MCAD (medium-chain acyl-CoA dehydrogenase) is an oxidoreductase enzyme of the mitochondrial fatty acid beta-oxidation pathway that is specific for acyl chain lengths of 4 to 16. It also utilizes the electron transfer flavoprotein (ETF) as electron acceptor that transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
Product was previously marketed under the MitoSciences sub-brand.
Alternative versions available:
Our Abpromise guarantee covers the use of ab110296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.125 µg/ml. Predicted molecular weight: 47 kDa.|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|In-Cell ELISA||Use a concentration of 1 µg/ml. (0.1 µg/well)|
|IHC-P||1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
HL-60 cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of fenofibrate (ab120832). Increased expression of ACADM in HL-60 cells correlates with an increase in fenofibrate concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab110296 at 1 µg/ml and ab9484 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 dilution and visualised using ECL development solution.