Anti-ACADM/MCAD antibody (ab13677)
Key features and details
- Goat polyclonal to ACADM/MCAD
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ACADM/MCAD antibody
See all ACADM/MCAD primary antibodies -
Description
Goat polyclonal to ACADM/MCAD -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ACADM/MCAD aa 400 to the C-terminus (C terminal).
Database link: P11310 -
Positive control
- Human Heart Lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).
A 1 hour primary incubation is recommended for this product. |
IHC-P |
Use a concentration of 3.75 µg/ml.
|
Notes |
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WB
Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa). A 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 3.75 µg/ml. |
Target
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Function
This enzyme is specific for acyl chain lengths of 4 to 16. -
Pathway
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
Involvement in disease
Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy. -
Sequence similarities
Belongs to the acyl-CoA dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 34 Human
- Omim: 607008 Human
- SwissProt: P11310 Human
- Unigene: 445040 Human
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Alternative names
- ACAD 1 antibody
- ACAD1 antibody
- Acadm antibody
see all
Images
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Anti-ACADM/MCAD antibody (ab13677) at 0.05 µg/ml + Human heart lysates at 35 µg
Developed using the ECL technique.
Predicted band size: 48 kDaAb13677 staining (0.05
µ g/ml) of Human Heart lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Primary incubated for 1 hour.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab13677 has been referenced in 2 publications.
- Wang F et al. PP56 improves energy homeostasis in a mouse model of pancreatic cancer. Tumour Biol 31:309-13 (2010). WB ; Mouse . PubMed: 20422342
- Powelka AM et al. Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes. J Clin Invest 116:125-36 (2006). WB ; Mouse . PubMed: 16374519