Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab13677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human ACADM peptide (ab23229).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionThis enzyme is specific for acyl chain lengths of 4 to 16.
  • PathwayLipid metabolism; mitochondrial fatty acid beta-oxidation.
  • Involvement in diseaseDefects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
  • Sequence similaritiesBelongs to the acyl-CoA dehydrogenase family.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAD 1 antibody
    • ACAD1 antibody
    • Acadm antibody
    • ACADM_HUMAN antibody
    • Acyl coenzyme A dehydrogenase antibody
    • Acyl coenzyme A dehydrogenase C 4 to C 12 straight chain antibody
    • FLJ18227 antibody
    • FLJ93013 antibody
    • FLJ99884 antibody
    • MCAD antibody
    • MCADH antibody
    • Medium chain acyl CoA dehydrogenase antibody
    • Medium chain fatty acyl CoA dehydrogenase antibody
    • Medium chain specific acyl CoA dehydrogenase antibody
    • Medium chain specific acyl CoA dehydrogenase mitochondrial antibody
    • Medium-chain specific acyl-CoA dehydrogenase antibody
    • mitochondrial antibody
    see all

Anti-ACADM antibody images

  • Anti-ACADM antibody (ab13677) at 0.05 µg/ml + Human heart lysates at 35 µg
    Developed using the ECL technique

    Predicted band size : 48 kDa


    Primary incubated for 1 hour.

  • ab13677 (3.8 µg/ml) staining ACADM in paraffin-embedded human Liver. Steamed antigen retieval with citrate buffer (pH 6) was carried out.

References for Anti-ACADM antibody (ab13677)

This product has been referenced in:
  • Wang F  et al. PP56 improves energy homeostasis in a mouse model of pancreatic cancer. Tumour Biol 31:309-13 (2010). WB ; Mouse . Read more (PubMed: 20422342) »
  • Powelka AM  et al. Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes. J Clin Invest 116:125-36 (2006). WB ; Mouse . Read more (PubMed: 16374519) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Neurons)
Specification Neurons
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

Submitted May 26 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"