Anti-ACADM antibody [EPR3708] (ab92461)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-ACADM antibody [EPR3708]
See all ACADM primary antibodies -
DescriptionRabbit monoclonal [EPR3708] to ACADM
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Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IF, Flow Cytmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (Human)
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Positive control
- WB: Human heart or fetal liver tissue lysates; HeLa, HepG2, or K562 cell lysates. IHC-P: Human liver tissue. ICC/IF: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3708
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab92461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | ||
| IP | ||
| IHC-P | ||
| ICC/IF | ||
| Flow Cyt |
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
IP: 1/10 - 1/100.
WB: 1/10000 - 1/50000. Predicted molecular weight: 47 kDa.
Is unsuitable for Flow Cyt.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionThis enzyme is specific for acyl chain lengths of 4 to 16.
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PathwayLipid metabolism; mitochondrial fatty acid beta-oxidation.
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Involvement in diseaseDefects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
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Sequence similaritiesBelongs to the acyl-CoA dehydrogenase family.
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Cellular localizationMitochondrion matrix.
- Information by UniProt
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Database links
- Entrez Gene: 34 Human
- Entrez Gene: 11364 Mouse
- Entrez Gene: 24158 Rat
- Omim: 607008 Human
- SwissProt: P11310 Human
- SwissProt: P45952 Mouse
- SwissProt: P08503 Rat
- Unigene: 445040 Human
see all -
Alternative names
- ACAD 1 antibody
- ACAD1 antibody
- Acadm antibody
see all
Anti-ACADM antibody [EPR3708] images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACADM antibody [EPR3708] (ab92461)](http://a.abcam.com/ps/products/92/ab92461/Images/ACADM-Primary-antibodies-ab92461-2.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACADM antibody [EPR3708] (ab92461)ab92461, at 1/100 dilution, staining ACADM in formalin-fixed, paraffin-embedded Human liver tissue by immunohistochemistry. -
![Immunocytochemistry/ Immunofluorescence - ACADM antibody [EPR3708] (ab92461)](http://a.abcam.com/ps/products/92/ab92461/Images/ACADM-Primary-antibodies-ab92461-3.JPG)
Immunocytochemistry/ Immunofluorescence - ACADM antibody [EPR3708] (ab92461)Immunofluorescent staining of ACADM in HeLa cells using ab92461 at 1/100 dilution. -
![Flow Cytometry - Anti-ACADM antibody [EPR3708] (ab92461)](http://a.abcam.com/ps/products/92/ab92461/Images/ab92461-268991-ab92461FC1.jpg)
Flow Cytometry - Anti-ACADM antibody [EPR3708] (ab92461)Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ACADM with unpurified ab92461 at 1/50 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
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![Western blot - ACADM antibody [EPR3708] (ab92461)](http://a.abcam.com/ps/products/92/ab92461/Images/ACADM-Primary-antibodies-ab92461-1.JPG)
Western blot - ACADM antibody [EPR3708] (ab92461)All lanes : Anti-ACADM antibody [EPR3708] (ab92461) at 1/10000 dilution
Lane 1 : Human heart lysate
Lane 2 : fetal liver lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP conjugated Goat anti-Rabbit Ig at 1/2000 dilution
Predicted band size : 47 kDa
Protocols
References for Anti-ACADM antibody [EPR3708] (ab92461)
This product has been referenced in:
- Tan EP et al. Altering O-linked ß-N-acetylglucosamine cycling disrupts mitochondrial function. J Biol Chem 289:14719-30 (2014). WB . Read more (PubMed: 24713701) »
- Mells JE et al. Glp-1 analog, liraglutide, ameliorates hepatic steatosis and cardiac hypertrophy in C57BL/6J mice fed a Western diet. Am J Physiol Gastrointest Liver Physiol 302:G225-35 (2012). WB ; Mouse . Read more (PubMed: 22038829) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"