Recombinant
RabMAb

Anti-ACADM antibody [EPR3708] (ab92461)

Abreviews (3)Q&A (5)Specific References (3)

Overview

  • Product name
    Anti-ACADM antibody [EPR3708]
    See all ACADM primary antibodies
  • Description
    Rabbit monoclonal [EPR3708] to ACADM
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • WB: Human heart or fetal liver tissue lysates; HeLa, HepG2, or K562 cell lysates. IHC-P: Human liver tissue. ICC/IF: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab92461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
ICC/IF
Flow Cyt
  • Application notes
    ICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended.
    IP: 1/10 - 1/100.
    WB: 1/10000 - 1/50000. Predicted molecular weight: 47 kDa.

    Is unsuitable for Flow Cyt.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      This enzyme is specific for acyl chain lengths of 4 to 16.
    • Pathway
      Lipid metabolism; mitochondrial fatty acid beta-oxidation.
    • Involvement in disease
      Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy.
    • Sequence similarities
      Belongs to the acyl-CoA dehydrogenase family.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • ACAD 1 antibody
      • ACAD1 antibody
      • Acadm antibody
      • ACADM_HUMAN antibody
      • Acyl coenzyme A dehydrogenase antibody
      • Acyl coenzyme A dehydrogenase C 4 to C 12 straight chain antibody
      • FLJ18227 antibody
      • FLJ93013 antibody
      • FLJ99884 antibody
      • MCAD antibody
      • MCADH antibody
      • Medium chain acyl CoA dehydrogenase antibody
      • Medium chain fatty acyl CoA dehydrogenase antibody
      • Medium chain specific acyl CoA dehydrogenase antibody
      • Medium chain specific acyl CoA dehydrogenase mitochondrial antibody
      • Medium-chain specific acyl-CoA dehydrogenase antibody
      • mitochondrial antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM antibody [EPR3708] (ab92461)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADM antibody [EPR3708] (ab92461)
      ab92461, at 1/100 dilution, staining ACADM in formalin-fixed, paraffin-embedded Human liver tissue by immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-ACADM antibody [EPR3708] (ab92461)
      Immunocytochemistry/ Immunofluorescence - Anti-ACADM antibody [EPR3708] (ab92461)
      Immunofluorescent staining of ACADM in HeLa cells using ab92461 at 1/100 dilution.
    • Flow Cytometry - Anti-ACADM antibody [EPR3708] (ab92461)
      Flow Cytometry - Anti-ACADM antibody [EPR3708] (ab92461)

      Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ACADM with unpurified ab92461 at 1/50 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    • Western blot - Anti-ACADM antibody [EPR3708] (ab92461)
      Western blot - Anti-ACADM antibody [EPR3708] (ab92461)
      All lanes : Anti-ACADM antibody [EPR3708] (ab92461) at 1/10000 dilution

      Lane 1 : Human heart lysate
      Lane 2 : fetal liver lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti-Rabbit Ig at 1/2000 dilution

      Predicted band size: 47 kDa

    References

    This product has been referenced in:
    • Tan EP  et al. Altering O-linked ß-N-acetylglucosamine cycling disrupts mitochondrial function. J Biol Chem 289:14719-30 (2014). WB . Read more (PubMed: 24713701) »
    • Mells JE  et al. Glp-1 analog, liraglutide, ameliorates hepatic steatosis and cardiac hypertrophy in C57BL/6J mice fed a Western diet. Am J Physiol Gastrointest Liver Physiol 302:G225-35 (2012). WB ; Mouse . Read more (PubMed: 22038829) »

    See all 3 Publications for this product

    Publishing research using ab92461? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    8 reviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-ACADM antibody [EPR3708]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human hepatocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    Specification
    human hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    Abcam user community

    Verified customer

    Submitted Dec 28 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ACADM antibody [EPR3708]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293)
    Specification
    HEK293
    Blocking step
    Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Fixative
    Formaldehyde
    Read More
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    Abcam user community

    Verified customer

    Submitted Apr 03 2017

    Western blot abreview for Anti-ACADM antibody [EPR3708]

     Excellent
    Abreviews
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10% SDS PAGE)
    Sample
    Mouse Tissue lysate - whole (Brain)
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
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    Username

    Abcam user community

    Verified customer

    Submitted May 27 2014

    I have isolated mitochondrial, cytosolic and nuclear fraction using standard protocol, also repeated the western blotting with ab92461. I am herewith attaching my result. Band is present through out the sample. Intensity of the band is comparatively hi...

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    I appreciate the time that has been spent on these experiments and would be pleased to arrange, in compensation, a replacement of ab92461 with another anti-ACADM such as ab110296. Alternatively I can also arrange a credit note. I look forward to hea...

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    Kindly solve the problem with ab92461 antibody and also help me to save my time

    Thank you very much for your email. My colleague is in contact with the laboratory in order to try to find out what the problem might is. Indeed we have to investigate this issue further as the quality of our products is most important. We have had no ...

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    I am herewith attaching images of Western blot of ACADM and Beta actin. Please compare these  Regards

    Thank you for sending the image. We can’t make assumptions for the specificity of an antibody because of its same size band specificity of as of beta actin. Many protein have similar sizes, so experimental evidence is always good to prove the cross...

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    Subject - enquiry regarding ab92461 [CCE3243834] Thank you very much for your reply. I have repeated the procedure and developed band for ACADM and Beta actin using ECL. It is found that Anti-ACADM detected the Beta actin band. That is why it showed ba...

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    Thank you for contacting us. The antibody is specific against ACADM protein only; ab92461 should not recognize the beta actin protein. Could you please explain why you think the antibody is detecting the beta actin protein? In regards to the exp...

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    LOT NUMBER GR 15062-5 ORDER NUMBER 2117 DESCRIPTION OF THE PROBLEM Non-specific band SAMPLE Total protein from rat heart tissue PRIMARY ANTIBODY Abcam Rabbut monoclonal to ACADM (ab92461), 1:10000 dilution at 5% BSA at TBS-T Buffer at 4Degree , overnig...

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    Thank you for your enquiry regarding ab92461 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. I appreciate the time you have spent on these experimen...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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