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|Sample type||Average %||Range|
|Serum||104||95% - 118%|
|Cell culture media||105||97% - 114%|
ab129734 Medium-chain-acyl-CoA dehydrogenase (MCAD, gene ACADM) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the accurate quantitative measurement of medium-chain-acyl-CoA dehydrogenase in human, rat and mouse tissue and cell culture extracts. The assay employs an ACADM specific antibody coated onto well plate strips. Standards and samples are pipetted into the wells and ACADM present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a primary anti-ACADM detector antibody is added. After washing away unbound detector antibody, an HRP-conjugated secondary detector antibody (HRP Label) specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ACADM bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
MCAD (medium-chain acyl-CoA dehydrogenase), encoded by the ACADM gene, is an oxidoreductase enzyme of the mitochondrial fatty acid beta-oxidation pathway that is specific for acyl chain lengths of 4 to 16. MCAD utilizes the electron transfer flavoprotein (ETF) as an electron acceptor that transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).
|Components||1 x 96 tests|
|10X ACADM Detector Antibody||1 x 1ml|
|10X Blocking Buffer||1 x 6ml|
|10X HRP Label||1 x 1ml|
|20X Buffer||1 x 20ml|
|ACADM Microplate (12 x 8 antibody coated well strips)||96 tests|
|ACADM Standard||1 vial|
|Extraction Buffer||1 x 15ml|
|HRP Development Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab129734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|