MitoSciences (MS784)

Anti-ACADS antibody [7E1AB5 ] (ab110318)

Overview

  • Product nameAnti-ACADS antibody [7E1AB5 ]See all ACADS primary antibodies ...
  • Description
    Mouse monoclonal [7E1AB5 ] to ACADS
  • Tested applicationsWB, ICC/IF, In-Cell ELISA, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein Human ACADS

  • Positive control
    • Fibroblast, HeLa, HepG2. Human Heart FFPE tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab110318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa.
ICC/IF Use a concentration of 1 µg/ml.
In-Cell ELISA Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

  • PathwayLipid metabolism; mitochondrial fatty acid beta-oxidation.
  • Involvement in diseaseDefects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD) [MIM:201470]. It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults.
  • Sequence similaritiesBelongs to the acyl-CoA dehydrogenase family.
  • Cellular localizationMitochondrion matrix.
  • Target information above from: UniProt accession P16219 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ACAD3 antibody
    • ACADS antibody
    • ACADS_HUMAN antibody
    • Acyl Coenzyme A dehydrogenase, C2 to C3 short chain antibody
    • Acyl-CoA dehydrogenase, C2 to C3 short chain antibody
    • Acyl-CoA dehydrogenase, short chain antibody
    • Acyl-Coenzyme A dehydrogenase, short chain antibody
    • AI196007 antibody
    • Bcd-1 antibody
    • Bcd1 antibody
    • Butyryl CoA dehydrogenase antibody
    • Butyryl-CoA dehydrogenase antibody
    • EC 1.3.99.2 antibody
    • mitochondrial antibody
    • SCAD antibody
    • Short chain acyl CoA dehydrogenase antibody
    • Short-chain specific acyl-CoA dehydrogenase antibody
    • Short-chain specific acyl-CoA dehydrogenase, mitochondrial antibody
    • Unsaturated acyl CoA reductase antibody
    see all

Anti-ACADS antibody [7E1AB5 ] images

  • All lanes : Anti-ACADS antibody [7E1AB5 ] (ab110318) at 1 µg/ml

    Lane 1 : HepG2 whole cell cells at 10 µg
    Lane 2 : ACADS at 0.01 µg


    Predicted band size : 44 kDa
  • Immunocytochemistry image of stained human fibroblasts. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110318 (1 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix.
  • IHC image of ACADS staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110318, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ACADS antibody [7E1AB5 ] (ab110318)

ab110318 has not yet been referenced specifically in any publications.

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