Recombinant
RabMAb

Anti-ACADS antibody [EPR10862(B)] (ab156571)

Overview

  • Product name
    Anti-ACADS antibody [EPR10862(B)]
    See all ACADS primary antibodies
  • Description
    Rabbit monoclonal [EPR10862(B)] to ACADS
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human ACADS (P16219).

  • Positive control
    • fetal liver and fetal heart lysates, Human heart and Human thyroid gland tissues
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Associated products

Applications

Our Abpromise guarantee covers the use of ab156571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 44 kDa).
IHC-P 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Pathway
      Lipid metabolism; mitochondrial fatty acid beta-oxidation.
    • Involvement in disease
      Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD) [MIM:201470]. It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults.
    • Sequence similarities
      Belongs to the acyl-CoA dehydrogenase family.
    • Cellular localization
      Mitochondrion matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAD3 antibody
      • ACADS antibody
      • ACADS_HUMAN antibody
      • Acyl Coenzyme A dehydrogenase, C2 to C3 short chain antibody
      • Acyl-CoA dehydrogenase, C2 to C3 short chain antibody
      • Acyl-CoA dehydrogenase, short chain antibody
      • Acyl-Coenzyme A dehydrogenase, short chain antibody
      • AI196007 antibody
      • Bcd-1 antibody
      • Bcd1 antibody
      • Butyryl CoA dehydrogenase antibody
      • Butyryl-CoA dehydrogenase antibody
      • EC 1.3.99.2 antibody
      • mitochondrial antibody
      • SCAD antibody
      • Short chain acyl CoA dehydrogenase antibody
      • Short-chain specific acyl-CoA dehydrogenase antibody
      • Short-chain specific acyl-CoA dehydrogenase, mitochondrial antibody
      • Unsaturated acyl CoA reductase antibody
      see all

    Images

    • ab156571 showing +ve staining in Human normal liver.
    • ab156571 showing +ve staining in Human gastric adenocarcinoma.
    • ab156571 showing +ve staining in Human normal colon.
    • ab156571 showing +ve staining in Human urinary bladder carcinoma.
    • All lanes : Anti-ACADS antibody [EPR10862(B)] (ab156571) at 1/1000 dilution

      Lane 1 : fetal liver lysate
      Lane 2 : fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 44 kDa
      Observed band size: 41 kDa (why is the actual band size different from the predicted?)

    • Immunoprecipitation. ab156571 at 1/1000 staining ACADS in fetal liver lysate immunoprecipitated using ab156571 at 1/10. Predicted band size : 41 kDa.
    • Immunohistochemical analysis of paraffin embedded Human heart tissue labeling ACADS with ab156571 antibody at 1/50.
    • Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue labeling ACADS with ab156571 antibody at 1/50.

    References

    This product has been referenced in:
    • Zeng Z  et al. Effects of short-chain acyl-CoA dehydrogenase on cardiomyocyte apoptosis. J Cell Mol Med 20:1381-91 (2016). WB ; Rat . Read more (PubMed: 26989860) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Heart (Embryonic))
    Permeabilization
    Yes - Tween-20
    Specification
    Heart (Embryonic)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 29 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 19 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up