Overview

  • Product name
  • Description
    Rabbit polyclonal to ACADVL
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 18-341 of Human ACADVL (P49748).

  • Positive control
    • H1299, HeLa, HepG2 and mouse liver whole cell lysates, HeLa cells, H358 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab155138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 70 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accomodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons.
  • Pathway
    Lipid metabolism; mitochondrial fatty acid beta-oxidation.
  • Involvement in disease
    Defects in ACADVL are the cause of acyl-CoA dehydrogenase very long chain deficiency (ACADVLD) [MIM:201475]. ACADVLD is an autosomal recessive disease which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form, with early onset, high mortality, and high incidence of cardiomyopathy; a milder childhood form, with later onset, usually with hypoketotic hypoglycemia as the main presenting feature, low mortality, and rare cardiomyopathy; and an adult form, with isolated skeletal muscle involvement, rhabdomyolysis, and myoglobinuria, usually triggered by exercise or fasting.
  • Sequence similarities
    Belongs to the acyl-CoA dehydrogenase family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAD 6 antibody
    • ACAD6 antibody
    • ACADV_HUMAN antibody
    • Acadvl antibody
    • Acyl CoA dehydrogenase very long chain antibody
    • Acyl Coenzyme A dehydrogenase very long chain antibody
    • LCACD antibody
    • mitochondrial antibody
    • Very long chain specific acyl CoA dehydrogenase antibody
    • Very long chain specific acyl CoA dehydrogenase mitochondrial antibody
    • Very long-chain specific acyl-CoA dehydrogenase antibody
    • VLCAD antibody
    see all

Images

  • Anti-ACADVL antibody (ab155138) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Anti-ACADVL antibody (ab155138) at 1/3000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 70 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling ACADVL with ab155138 at 1/200. Image at the bottom costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded H358 xenograft labeling ACADVL with ab155138 antibody at 1/250.

References

This product has been referenced in:
  • Yu J  et al. High-throughput proteomics integrated with gene microarray for discovery of colorectal cancer potential biomarkers. Oncotarget 7:75279-75292 (2016). IHC-P ; Human . Read more (PubMed: 27661117) »
  • LaBarge SA  et al. p300 is not required for metabolic adaptation to endurance exercise training. FASEB J 30:1623-33 (2016). Read more (PubMed: 26712218) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 28 2017

Application
Western blot
Sample
Rat Cell lysate - other (Whole cell lysate of rat ST14a cells, and mitochon)
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE, Laemmli buffer with 10% β-Mercaptoethanol,10 min at 55C denaturation.)
Loading amount
20 µg
Specification
Whole cell lysate of rat ST14a cells, and mitochon
Blocking step
SEA Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Svitlana Yablonska

Verified customer

Submitted Aug 07 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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