Anti-ACAT1 antibody (ab154396)
Key features and details
- Rabbit polyclonal to ACAT1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ACAT1 antibody
See all ACAT1 primary antibodies -
Description
Rabbit polyclonal to ACAT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment corresponding to Human ACAT1 aa 1-151.
Database link: P24752 -
Positive control
- WB: SW480, HepG2, Jurkat, HEK-293T, A431 and Molt-4 whole cell lysate. Mouse brain whole cell lysate. ICC/IF: HeLa and A431 cells. IHC-P: Human normal kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Plays a major role in ketone body metabolism. -
Involvement in disease
Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype. -
Sequence similarities
Belongs to the thiolase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 511082 Cow
- Entrez Gene: 38 Human
- Entrez Gene: 110446 Mouse
- Omim: 607809 Human
- SwissProt: Q29RZ0 Cow
- SwissProt: P24752 Human
- SwissProt: Q8QZT1 Mouse
- Unigene: 232375 Human
see all -
Alternative names
- ACAT 1 antibody
- ACAT antibody
- acat1 antibody
see all
Images
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All lanes : Anti-ACAT1 antibody (ab154396) at 1/500 dilution
Lane 1 : Wild-type SW480 cell lysate
Lane 2 : ACAT1 CRISPR/Cas9 edited SW480 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 45 kDaLanes 1 - 4: Merged signal (red and green). Green - ab154396 observed at 45 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab154396 was shown to react with ACAT1 in western blot. The band observed in the knockout cell line ab269621 (knockout cell lysate ab272431) lane below 45 kDa is likely to represent a truncated form. This has not been investigated further. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab154396 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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A431 cells stained for ACAT1 using ab154396 at 1/1000 dilution in ICC/IF.
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Paraffin embedded human normal kidney tissue stained for ACAT1 using ab154396 at 1/1000 dilution in immunohistochemical analysis.
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HeLa cells stained for ACAT1 using ab154396 at 1/1000 dilution in ICC/IF.
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All lanes : Anti-ACAT1 antibody (ab154396) at 1/500 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : Molt-4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
10% SDS PAGE -
Anti-ACAT1 antibody (ab154396) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 45 kDa
10% SDS PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154396 has been referenced in 1 publication.
- Liu Z et al. Correlation of cholesteryl ester metabolism to pathogenesis, progression and disparities in colorectal Cancer. Lipids Health Dis 21:22 (2022). PubMed: 35172832