Overview

  • Product name
  • Description
    Goat polyclonal to ACAT1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Synthetic peptide:

    C-DEEYKRVDFSKVPK

    , corresponding to internal sequence amino acids 253-266 of Human ACAT1 (NP_000010.1).

  • Positive control
    • Human, Mouse and Rat Liver lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab99190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

Target

  • Function
    Plays a major role in ketone body metabolism.
  • Involvement in disease
    Defects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.
  • Sequence similarities
    Belongs to the thiolase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAT 1 antibody
    • ACAT antibody
    • acat1 antibody
    • Acetoacetyl CoA thiolase antibody
    • acetoacetyl Coenzyme A thiolase antibody
    • Acetoacetyl-CoA thiolase antibody
    • Acetyl CoA acetyltransferase, mitochondrial antibody
    • Acetyl Coenzyme A acetyltransferase 1 antibody
    • Acetyl-CoA acetyltransferase antibody
    • acetyl-coa acetyltransferase precursor, mitochondrial antibody
    • Acetyl-CoA thiolase, mitochondrial antibody
    • acetyl-Coenzyme A acetyltransferase 1 antibody
    • MAT antibody
    • mitochondrial acetoacetyl-CoA thiolase antibody
    • mitochondrial antibody
    • RATACAL antibody
    • T2 antibody
    • testicular tissue protein Li 198 antibody
    • THIL antibody
    • THIL_HUMAN antibody
    see all

Images

  • Anti-ACAT1 antibody (ab99190) at 0.1 µg/ml + Mouse liver lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 45 kDa
    Primary incubation was 1 hour.
  • All lanes : Anti-ACAT1 antibody (ab99190) at 0.1 µg/ml

    Lane 1 : Mouse liver lysates
    Lane 2 : Mouse liver lysates

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 45 kDa

References

ab99190 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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