The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
1/50 - 1/100. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Use at an assay dependent concentration. PubMed: 24658146
FunctionPlays a major role in ketone body metabolism.
Involvement in diseaseDefects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.
Sequence similaritiesBelongs to the thiolase family.
ab71407 at 1/50 dilution staining ACAT1 - C- terminal in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
References for Anti-ACAT1 antibody - C-terminal (ab71407)
This product has been referenced in:
Wang XQ et al. CLI-095 decreases atherosclerosis by modulating foam cell formation in apolipoprotein E-deficient mice. Mol Med Rep14:49-56 (2016).
Read more (PubMed: 27176130) »
Taha MS et al. Subcellular fractionation and localization studies reveal a direct interaction of the fragile X mental retardation protein (FMRP) with nucleolin. PLoS One9:e91465 (2014).
Read more (PubMed: 24658146) »