Overview

  • Product nameAnti-ACAT1 antibody - C-terminal
    See all ACAT1 primary antibodies
  • Description
    Rabbit polyclonal to ACAT1 - C-terminal
  • Tested applicationsSuitable for: IHC-P, ELISA, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C-terminal region of human ACAT1 conjugated to KLH

  • Positive control
    • SK-BR-3 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab71407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
ELISA 1/1000.
WB 1/50 - 1/100. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 24658146

Target

  • FunctionPlays a major role in ketone body metabolism.
  • Involvement in diseaseDefects in ACAT1 are a cause of 3-ketothiolase deficiency (3KTD) [MIM:203750]; also known as alpha-methylacetoaceticaciduria. 3KTD is an inborn error of isoleucine catabolism characterized by intermittent ketoacidotic attacks associated with unconsciousness. Some patients die during an attack or are mentally retarded. Urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, triglylglycine, butanone is increased. It seems likely that the severity of this disease correlates better with the environmental or acquired factors than with the ACAT1 genotype.
  • Sequence similaritiesBelongs to the thiolase family.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACAT 1 antibody
    • ACAT antibody
    • acat1 antibody
    • Acetoacetyl CoA thiolase antibody
    • acetoacetyl Coenzyme A thiolase antibody
    • Acetoacetyl-CoA thiolase antibody
    • Acetyl CoA acetyltransferase, mitochondrial antibody
    • Acetyl Coenzyme A acetyltransferase 1 antibody
    • Acetyl-CoA acetyltransferase antibody
    • acetyl-coa acetyltransferase precursor, mitochondrial antibody
    • Acetyl-CoA thiolase, mitochondrial antibody
    • acetyl-Coenzyme A acetyltransferase 1 antibody
    • MAT antibody
    • mitochondrial acetoacetyl-CoA thiolase antibody
    • mitochondrial antibody
    • RATACAL antibody
    • T2 antibody
    • testicular tissue protein Li 198 antibody
    • THIL antibody
    • THIL_HUMAN antibody
    see all

Anti-ACAT1 antibody - C-terminal images

  • Anti-ACAT1 antibody - C-terminal (ab71407) at 1/60 dilution + SK-BR-3 cell line lysate at 35 µg

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 130 kDa. We are unsure as to the identity of these extra bands.
  • ab71407 at 1/50 dilution staining ACAT1 - C- terminal in human hepatocarcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References for Anti-ACAT1 antibody - C-terminal (ab71407)

This product has been referenced in:
  • Wang XQ  et al. CLI-095 decreases atherosclerosis by modulating foam cell formation in apolipoprotein E-deficient mice. Mol Med Rep 14:49-56 (2016). Read more (PubMed: 27176130) »
  • Taha MS  et al. Subcellular fractionation and localization studies reveal a direct interaction of the fragile X mental retardation protein (FMRP) with nucleolin. PLoS One 9:e91465 (2014). ICC/IF ; Human . Read more (PubMed: 24658146) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"