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Western blot - ACAT2 antibody (ab60282)
Western blot - ACAT2 antibody (ab60282)
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Product Name 

ACAT2 antibody

 

See all ACAT2 antibodies (4)...

Product type 

Primary antibodies

Description 

Chicken polyclonal to ACAT2

Immunogen 

Synthetic peptide, corresponding to amino acids 161/250 of Human ACAT2.

Reacts with 
(species key)

Hu

Epitope 

Amino acids 161-250.

Tested applications 
(see key)

WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
WB: 1/2000. Predicted molecular weight: 42 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism

Relevance 

Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.

 

Alternative names ACAT2 antibody (ab60282)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

39    (Human)

Omim

    

100678    (Human)

SwissProt

    

Q9BWD1    (Human)

Raised in 

Chicken

Clonality 

Polyclonal

Isotype 

IgY

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: PBS

Form 

Liquid

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ACAT2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for ACAT2 antibody images, references, products related to ab60282 and other tools.

 

ACAT2 antibody images:

Product image  Western blot - ACAT2 antibody (ab60282)

Western blot - ACAT2 antibody (ab60282)

ACAT2 antibody (ab60282) at 1/2000 dilution + E coli-derived fusion protein

Secondary
Goat anti-IgY-HRP at 1/1000 dilution

Predicted band size : 42 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)


Colorimetric method used for signal development. The observed band does not match the predicted band size as the test antigen is a fusion protein.

 
 

Search PubMed (MEDLINE) for references to ACAT2

 

ACAT2 antibody - more information

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Lysates

 

Compatible IgY Secondaries for ACAT2 antibody

ab6749

  

Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (FITC)

ab6752

  

Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin)

ab6753

  

Rabbit polyclonal Secondary Antibody to Chicken IgY - H&L (HRP)

ab6877

  

Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP)

ab7117

  

Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin), pre-adsorbed

 

Other ACAT2 Antibodies

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