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Western blot - ACAT2 antibody (ab66259)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACAT2 antibody (ab66259)
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Product Name
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ACAT2 antibody
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See all ACAT2 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to ACAT2
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Immunogen
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Synthetic peptide: SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK, corresponding to a region within internal sequence amino acids 145-194 of Human ACAT2
BLAST 'SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution. Titre using peptide based assay 1/12500.
IHC-P: Use at a concentration of 4.0 - 8.0 µg/ml.
WB: Use at a concentration of 1.0 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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K562 cell lysate. Human liver tissue.
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Cellular localization
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Cytoplasmic
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Research areas
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Cardiovascular >> Atherosclerosis >> Lipoprotein metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
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Relevance
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Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.
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Database links
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The links below go to external sites and will open in a new browser window Entrez Gene | | 39 (Human)
| Omim | | 100678 (Human)
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this ACAT2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for ACAT2 antibody images, references, products related to ab66259 and other tools.
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ACAT2 antibody images:
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 Western blot - ACAT2 antibody (ab66259)
ACAT2 antibody (ab66259) at 1 µg/ml + K562 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 41 kDa
Observed band size : 41 kDa
Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACAT2 antibody (ab66259)
ab66259, at 4.0 µg/ml, staining ACAT2 in Human liver tissue using paraffin-embedded tissue. The arrows indicate positively stained hepatocytes.
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Search PubMed (MEDLINE) for references to ACAT2
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ACAT2 antibody - more information
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Customer reviews (feedback) regarding ACAT2 antibody |
Customer FAQs regarding ACAT2 antibody |
Protocols for ACAT2 antibody |
Price and availability of products related to ACAT2 antibody |
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ACAT2 antibody - related products:
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ACAT2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about ACAT2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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