Overview

  • Product nameAnti-ACAT2 antibody
    See all ACAT2 primary antibodies
  • Description
    Rabbit polyclonal to ACAT2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human ACAT2.

    (Peptide available as ab91442.)

  • Positive control
    • This antibody gave a positive signal in human liver tissue lysate as well as the following whole cell lysates: HepG2; HEK293; K562.

Properties

Applications

Our Abpromise guarantee covers the use of ab74901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

  • Sequence similaritiesBelongs to the thiolase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acat2 antibody
    • acetoacetyl CoA thiolase antibody
    • acetoacetyl Coenzyme A thiolase antibody
    • Acetyl CoA acetyltransferase antibody
    • Acetyl CoA transferase like protein antibody
    • acetyl Coenzyme A acetyltransferase 2 antibody
    • Acetyl-CoA acetyltransferase antibody
    • Acetyl-CoA transferase-like protein antibody
    • Acyl Coenzyme A cholesterol acyltransferase 2 antibody
    • Cytosolic acetoacetyl-CoA thiolase antibody
    • cytosolic antibody
    • SOAT2 antibody
    • THIC_HUMAN antibody
    see all

Anti-ACAT2 antibody images

  • All lanes : Anti-ACAT2 antibody (ab74901) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa


    Exposure time : 90 seconds
  • ICC/IF image of ab74901 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74901, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) Hek293 cells at 5µg/ml.

References for Anti-ACAT2 antibody (ab74901)

ab74901 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab74901.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"