Anti-ACAT2 antibody (ab74901)

Overview

• Product nameAnti-ACAT2 antibodySee all ACAT2 primary antibodies ...
• Description
  Rabbit polyclonal to ACAT2
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Orangutan
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human ACAT2.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab91442.)

• Positive controlThis antibody gave a positive signal in human liver tissue lysate as well as the following whole cell lysates: HepG2; HEK293; K562.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Cholesterol Metabolism

  • Metabolism
  • Types of disease
  • Heart disease

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipoprotein metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Cholesterol Metabolism

  • Cardiovascular
  • Atherosclerosis
  • Lipoprotein metabolism

Applications

Target

• Sequence similaritiesBelongs to the thiolase family.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession Q9BWD1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 39 Human
  • Omim: 100678 Human
  • SwissProt: Q9BWD1 Human
  • Unigene: 571037 Human

• Alternative names
  Acat2 antibodyacetoacetyl CoA thiolase antibodyacetoacetyl Coenzyme A thiolase antibody

  Acetyl CoA acetyltransferase antibodyAcetyl CoA transferase like protein antibodyacetyl Coenzyme A acetyltransferase 2 antibodyAcetyl-CoA acetyltransferase antibodyAcetyl-CoA transferase-like protein antibodyAcyl Coenzyme A cholesterol acyltransferase 2 antibodyCytosolic acetoacetyl-CoA thiolase antibodycytosolic antibodySOAT2 antibodyTHIC_HUMAN antibody

  see all

Anti-ACAT2 antibody images

• 

  Western blot - ACAT2 antibody (ab74901)
  All lanes : Anti-ACAT2 antibody (ab74901) at 1 µg/ml
  
  Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
  Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
  Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 41 kDa
  Observed band size : 41 kDa
  
  
  Exposure time : 90 seconds
• 

  Immunocytochemistry/ Immunofluorescence - ACAT2 antibody (ab74901)
  ICC/IF image of ab74901 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74901, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) Hek293 cells at 5µg/ml.