The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionCation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.
Tissue specificityBrain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary.
Sequence similaritiesBelongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN1 subfamily.
Cellular localizationCell membrane. Localized at the plasma membrane of neurons, in the soma and punctated peripheral processes.