Overview

  • Product name
  • Description
    Rabbit polyclonal to ACCN2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (ELKAEEEEVG GVQPVSIQAF ASSSTLHGLA HIFSYERLSL KRALWALCFL) of Human ACCN2 (NP_064423).

  • Positive control
    • HT1080 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. (Isoform 1) Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.
  • Tissue specificity
    Expressed in most or all neurons.
  • Sequence similarities
    Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. ACCN2 subfamily.
  • Post-translational
    modifications
    Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel.
  • Cellular localization
    Cell membrane. Localizes in synaptosomes at dendritic synapses of neurons. Colocalizes with DLG4.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACCN2 antibody
    • ACCN2_HUMAN antibody
    • Acid sensing ion channel 1 antibody
    • Acid sensing ion channel 1a protein antibody
    • Acid sensing ion channel antibody
    • Acid-sensing ion channel 1 antibody
    • AI843610 antibody
    • Amiloride sensitive cation channel 2 neuronal antibody
    • Amiloride-sensitive cation channel 2 antibody
    • ASIC antibody
    • ASIC1 antibody
    • ASIC1A antibody
    • B530003N02Rik antibody
    • BNaC2 antibody
    • Brain sodium channel 2 antibody
    • Cation channel amiloride sensitive neuronal 2 antibody
    • hBNaC2 antibody
    • neuronal antibody
    see all

Images

  • Anti-ACCN2 antibody (ab94753) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Gel concentration: 12%


  • Predicted band size : 65 kDa
    Western Blot using ab94753 at 1.0ug/ml. Sample type: Hum. Adult Placenta Lysate


  • Predicted band size : 65 kDa
    Western Blot using ab94753 at 1.0ug/ml. Sample type: Hum. Fetal Heart Lysate


  • Predicted band size : 65 kDa
    Western Blot using ab94753 at 1.0ug/ml. Sample type: Hum. Fetal Liver Lysate


  • Predicted band size : 65 kDa
    Western Blot using ab94753 at 1.0ug/ml. Sample type: Hum. Fetal Lung Lysate


  • Predicted band size : 65 kDa
    Western Blot using ab94753 at 1.0ug/ml. Sample type: Hum. Fetal Muscle Lysate

References

ab94753 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting Abcam. The ACCN2 antibody, ab94753 had not previously been tested in IF and so it is unknown as to whether this antibody would be suitable for this application or not. Based on the information that you provided, there a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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