 image](#Acetyl-Coenzyme-A-carboxylase-alpha-Primary-antibodies-ab78274-1.jpg)
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Products:Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
Overview
Product name
Anti-Acetyl Coenzyme A carboxylase alpha antibody [EP687Y]
See all Acetyl Coenzyme A carboxylase alpha products (5) ...
Description
Rabbit monoclonal [EP687Y] to Acetyl Coenzyme A carboxylase alpha
Tested applications
Flow Cyt, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
KLH conjugated synthetic peptide corresponding to human acetyl Coenzyme A carboxylase alpha
Positive control
Lysates from A431 cells; human liver tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 60% Storage buffer (0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4)
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP687Y
Isotype
IgG1
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
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Western blot - acetyl Coenzyme A carboxylase alpha antibody (ab78274)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - acetyl Coenzyme A carboxylase alpha antibody (ab78274)
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Flow Cytometry-Anti-Acetyl Coenzyme A carboxylase alpha antibody [EP687Y](ab78274)
 image](/ps/datasheet/images/78/ab78274/Acetyl-Coenzyme-A-carboxylase-alpha-Primary-antibodies-ab78274-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab78274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/50
WB: 1/500 - 1/2000.Predicted molecular weight: 266 kDa.
IHC-P: 1/50
Target
Function
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
Tissue specificity
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
Pathway
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
Involvement in disease
Defects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:200350]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain.
Post-translational
modifications
Phosphorylation on Ser-1263 is required for interaction with BRCA1.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ13085
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACAC antibody
- ACACA antibody
- ACC alpha antibody
see all
Database links
- Entrez Gene: 31 Human
- Entrez Gene: 32 Human
- Entrez Gene: 107476 Mouse
- Entrez Gene: 60581 Rat
- Omim: 200350 Human
- SwissProt: O00763 Human
- SwissProt: Q13085 Human
- SwissProt: Q5SWU9 Mouse
see all
Anti-Acetyl Coenzyme A carboxylase alpha antibody [EP687Y] images:
Western blot - acetyl Coenzyme A carboxylase alpha antibody (ab78274)
Anti-Acetyl Coenzyme A carboxylase alpha antibody [EP687Y] (ab78274) at 1/2000 dilution + A431 cell lysate
Secondary
Goat anti-rabbit HRP conjugate at 1/5000 dilution
developed using the ECL technique
Predicted band size : 266 kDa
Observed band size : 266 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - acetyl Coenzyme A carboxylase alpha antibody (ab78274)
ab78274, at a 1/50 dilution, staining acetyl Coenzyme A carboxylase alpha in paraffin embedded human liver tissue by Immunohistochemistry.
Flow Cytometry-Anti-Acetyl Coenzyme A carboxylase alpha antibody [EP687Y](ab78274)
Overlay histogram showing HEK293 cells stained with ab78274 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78274, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-Acetyl Coenzyme A carboxylase alpha antibody [EP687Y] (ab78274)
ab78274 has not yet been referenced specifically in any publications.
Publishing research using ab78274? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"