acetyl Coenzyme A carboxylase alpha antibody [EP687Y] (ab78274)

Product Name 

acetyl Coenzyme A carboxylase alpha antibody [EP687Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP687Y] to acetyl Coenzyme A carboxylase alpha

Immunogen 

KLH conjugated synthetic peptide corresponding to human acetyl Coenzyme A carboxylase alpha

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: 1/50.
WB: 1/500 - 1/2000. Predicted molecular weight: 266 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Lysates from A431 cells; human liver tissue.

Cellular localization 

Cytoplasmic

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis

Relevance 

Acetyl Coenzyme A carboxylase alpha catalyzes the carboxylation of acetyl CoA to form malonyl CoA, the rate limiting step in the biogenesis of LCFA CoA. ACC1 carries three functions: biotin carboxyl carrier protein, biotin carboxylase, and carboxyltransferase (catalytic activity). Two variants of ACC1 have been described. One with 8 additional amino acids commencing at Pro 1196. The other which is 59aa shorter than the predominant fat and liver isoform exist in mammals. The presence of 8 additional amino acids inhibits the in vitro phosphorylation of the Ser1200 by cAMP dependent kinase. The two ACC1 isoforms are differentially regulated in a tissue specific manner and under different physiological conditions. The activity of ACC1 is finely regulated by hormone dependent phosphorylation and dephosphorylation.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP687Y

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 60% Storage buffer (0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4)

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

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