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Products:Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
Overview
Product name
Anti-Acetyl Coenzyme A carboxylase alpha antibody
See all Acetyl Coenzyme A carboxylase alpha products (5) ...
Description
Rabbit polyclonal to Acetyl Coenzyme A carboxylase alpha
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to a region between residue 2333 and the C-terminus (residue 2383) of human acetyl Coenzyme A carboxylase alpha (NP_942131.1).
Positive control
HeLa, 293T and NIH3T3 whole cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Synthesis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB: 1/2000 - 1/10000.Detects a band of approximately 266 kDa (predicted molecular weight: 266 kDa).
Application notes
Is unsuitable for or IP.
Target
Function
Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
Tissue specificity
Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
Pathway
Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
Involvement in disease
Defects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:200350]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain.
Post-translational
modifications
Phosphorylation on Ser-1263 is required for interaction with BRCA1.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ13085
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACAC antibody
- ACACA antibody
- ACC alpha antibody
see all
Database links
- Entrez Gene: 31 Human
- Entrez Gene: 32 Human
- Entrez Gene: 107476 Mouse
- Omim: 200350 Human
- SwissProt: O00763 Human
- SwissProt: Q13085 Human
- SwissProt: Q5SWU9 Mouse
- Unigene: 160556 Human
see all
Anti-Acetyl Coenzyme A carboxylase alpha antibody images:
Western blot - acetyl Coenzyme A carboxylase alpha antibody (ab72046)
All lanes : Anti-Acetyl Coenzyme A carboxylase alpha antibody (ab72046) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size : 266 kDa
Observed band size : 266 kDa
Additional bands at : 130 kDa,90 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Acetyl Coenzyme A carboxylase alpha antibody(ab72046)
IHC image of ab72046 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72046, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Acetyl Coenzyme A carboxylase alpha antibody (ab72046)
ab72046 has not yet been referenced specifically in any publications.
Publishing research using ab72046? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"