Anti-acetyl Lysine antibody [1C6] (ab22550)

Is this antibody has ever been tested in Immunoprecipitation?

ANSWER:

Thank you very much for your interest in ab22550. To our knowledge, ab22550 has not been tested in IP. Therefore, I can offer a discount off a future purchase if you buy ab22550 now, test it in IPand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab22550 in IP. I will then send a discount code. This code must be issued before purchasing ab22550 so please wait for my reply before ordering. 2. Purchase ab22550 either by phone, fax, or online (www.abcam.com). 3. Test it in IP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab22550 turns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

I would like to do some experiments to detect the lysine-specific acetylation of a non-histone protein. There is no sequence homology between the histone acetylation site used as the antigen to develop the antibody. Will the monoclonal acetylation antibody work to detect acetyl-lysine residues on any peptide?

ANSWER:

Thank you for your enquiry.

The synthetic peptide used to raise this antibody was a peptide sequence surrounding the acetylated lysine 9 of histone H3. This antibody has been shown to generate a ladder in a HeLa whole cell lysate demonstrating that it has the potential to recognise acetylated, non-histone proteins. I cannot guarantee that it will recognise every acetyl-lysine epitope given that each epitope will vary dependent on the adjacent residues. May I suggest that you consider our monoclonal N-epsilon acetyl Lysine antibody [11A1] (ab409). Mike Schutkowski has left a favourable review following it application against various acetylated lysine peptides.

Please do not hesitate to contact me should you require further assistance.

Do you have any information aboutthe ability of this antibody to react with other non-histone acetylated proteins?

ANSWER:

Thank you for your enquiry.

Yes, by Western blot, this antibody detects a ladder of acetylated proteins in HeLa cell lysates. In immunofluorescence procedures, this antibody recognizes acetylated lysine in gamma irradiated HeLa cells.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information,