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Product Name
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acetyl Lysine antibody [1C6]
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See all acetyl Lysine antibodies (9)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [1C6] to acetyl Lysine
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Immunogen
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Synthetic peptide: sequence surrounding the acetylated lysine 9 of histone H3
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab22550 recognises proteins with acetylated lysine.
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IF: Use at a concentration of 2 - 4 µg/ml.
WB: Use at a concentration of 1 µg/ml.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cell lysate.
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Research areas
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Chromatin and Nuclear Signaling >> Histones >> H2A >> Acetylated
Chromatin and Nuclear Signaling >> Histones >> H2B >> Acetylated
Chromatin and Nuclear Signaling >> Histones >> H4 >> Acetylated
Chromatin and Nuclear Signaling >> Histones >> H3 >> Acetylated
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Relevance
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In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.
The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.
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acetyl Lysine antibody [1C6] (ab22550) |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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1C6
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: PBS, 1mg/ml BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this acetyl Lysine antibody [1C6] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to acetyl Lysine
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acetyl Lysine antibody [1C6] - more information
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