acetyl Lysine antibody (HRP) (ab23364)

Product Name 

acetyl Lysine antibody (HRP)

Product type 

Primary antibodies

Description 

Rabbit polyclonal to acetyl Lysine (HRP)

Immunogen 

Acetylated KLH conjugates.

Reacts with 
(species key)

N/A

Specificity 

This antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP, no reaction to the non-acetylated proteins.

Tested applications 
(see key)

ELISA, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Microarray: Use at an assay dependent dilution.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Chromatin and Nuclear Signaling >> Histones >> H2A >> Acetylated
Chromatin and Nuclear Signaling >> Histones >> H2B >> Acetylated
Chromatin and Nuclear Signaling >> Histones >> H4 >> Acetylated
Chromatin and Nuclear Signaling >> Histones >> H3 >> Acetylated
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> Acetyl Lysine

Relevance 

In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.

The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.

The purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct label of primary anti-AcK will avoid the use of secondary antiboides therefore eliminating the interference of the 2nd antibody-conjugates.

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, 0.1M PBS, pH 7

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Conjugation 

Horse Radish Peroxidase

Concentration 

0.250 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

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