Anti-Acetyl Coenzyme A carboxylase alpha antibody (ab72046)

Overview

  • Product nameAnti-Acetyl Coenzyme A carboxylase alpha antibody
    See all Acetyl Coenzyme A carboxylase alpha primary antibodies
  • Description
    Rabbit polyclonal to Acetyl Coenzyme A carboxylase alpha
  • Tested applicationsSuitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Cow, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 2333 and the C-terminus (residue 2383) of human acetyl Coenzyme A carboxylase alpha (NP_942131.1).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Applications

Our Abpromise guarantee covers the use of ab72046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 266 kDa (predicted molecular weight: 266 kDa).
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionCatalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
    • Tissue specificityExpressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
    • PathwayLipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
    • Involvement in diseaseDefects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:200350]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.
    • Sequence similaritiesContains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • Post-translational
      modifications
      Phosphorylation on Ser-1263 is required for interaction with BRCA1.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAC antibody
      • ACACA antibody
      • ACACA_HUMAN antibody
      • ACC alpha antibody
      • ACC-alpha antibody
      • ACC1 antibody
      • ACCA antibody
      • Acetyl Coenzyme A antibody
      • Biotin carboxylase antibody
      see all

    Anti-Acetyl Coenzyme A carboxylase alpha antibody images

    • All lanes : Anti-Acetyl Coenzyme A carboxylase alpha antibody (ab72046) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 266 kDa
      Observed band size : 266 kDa
      Additional bands at : 130 kDa,90 kDa. We are unsure as to the identity of these extra bands.
    • IHC image of ab72046 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72046, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References for Anti-Acetyl Coenzyme A carboxylase alpha antibody (ab72046)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Sheep Tissue lysate - whole (Liver)
    Gel Running Conditions Reduced Denaturing (4-20% Tris Glycine Gels)
    Loading amount 75 µg
    Specification Liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. John Odhiambo

    Verified customer

    Submitted Oct 14 2015

    Application Western blot
    Sample Pig Tissue lysate - other (Muscle)
    Loading amount 8 µg
    Specification Muscle
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 13 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"