Anti-Acetyl Coenzyme A carboxylase alpha antibody (ab72046)

Overview

  • Product name
    Anti-Acetyl Coenzyme A carboxylase alpha antibody
    See all Acetyl Coenzyme A carboxylase alpha primary antibodies
  • Description
    Rabbit polyclonal to Acetyl Coenzyme A carboxylase alpha
  • Tested applications
    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Cow, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 2333 and the C-terminus (residue 2383) of human acetyl Coenzyme A carboxylase alpha (NP_942131.1).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Applications

Our Abpromise guarantee covers the use of ab72046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 266 kDa (predicted molecular weight: 266 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • All lanes : Anti-Acetyl Coenzyme A carboxylase alpha antibody (ab72046) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg


      Predicted band size : 266 kDa
      Observed band size : 266 kDa
      Additional bands at : 130 kDa,90 kDa. We are unsure as to the identity of these extra bands.
    • IHC image of ab72046 staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72046, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References

    This product has been referenced in:
    • Sujishi T  et al. Sitagliptin can inhibit the development of hepatic steatosis in high-fructose diet-fed ob/ob mice. J Clin Biochem Nutr 57:244-53 (2015). WB . Read more (PubMed: 26566312) »
    • Rawson P  et al. Metabolic proteomics of the liver and mammary gland during lactation. J Proteomics 75:4429-35 (2012). WB ; Cow . Read more (PubMed: 22554911) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Sheep Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris Glycine Gels)
    Loading amount
    75 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. John Odhiambo

    Verified customer

    Submitted Oct 14 2015

    Application
    Western blot
    Sample
    Pig Tissue lysate - other (Muscle)
    Loading amount
    8 µg
    Specification
    Muscle
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 13 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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