Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] (ab109368)


  • Product nameAnti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971]
    See all Acetyl Coenzyme A carboxylase alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR4971] to Acetyl Coenzyme A carboxylase alpha
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    corresponding to Human Acetyl Coenzyme A carboxylase alpha aa 1-100 (N terminal).

  • Positive control
    • HepG2, and SH-SY5Y cell lysates. Human brain tissue and Human skeletal muscle tissue. 293T cells.
  • General notes

     Alternative versions available:

    Anti-Acetyl Coenzyme A carboxylase alpha antibody (Alexa Fluor® 488) [EPR4971] (ab203994)

    Mouse and rat:  We have preliminary internal testing data to indicate this antibody may not react with these species, Please contact us for more information. 



    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.


Our Abpromise guarantee covers the use of ab109368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 266 kDa.

For unpurified use at 1/1000- 1/10000.

IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionCatalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
    • Tissue specificityExpressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
    • PathwayLipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
    • Involvement in diseaseDefects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:200350]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.
    • Sequence similaritiesContains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • Post-translational
      Phosphorylation on Ser-1263 is required for interaction with BRCA1.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAC antibody
      • ACACA antibody
      • ACACA_HUMAN antibody
      • ACC alpha antibody
      • ACC-alpha antibody
      • ACC1 antibody
      • ACCA antibody
      • Acetyl Coenzyme A antibody
      • Biotin carboxylase antibody
      see all

    Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] images

    • Overlay histogram showing SH-SY5Y cells stained with ab109368 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109368, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] (ab109368) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 266 kDa
    • Immunofluorescent staining of 293 cells using ab109368 at 1/100 dilution
    • Immunohistochemical analysis of paraffin-embedded skeletal muscle tissue using ab109368 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded brain tissue using ab109368 at 1/250 dilution.

    References for Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] (ab109368)

    ab109368 has not yet been referenced specifically in any publications.

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