Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] (ab109368)

Overview

  • Product nameAnti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971]
    See all Acetyl Coenzyme A carboxylase alpha primary antibodies
  • Description
    Rabbit monoclonal [EPR4971] to Acetyl Coenzyme A carboxylase alpha
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues at the N-terminus of Human Acetyl Coenzyme A carboxylase alpha

  • Positive control
    • HepG2, and SH-SY5Y cell lysates. Human brain tissue and Human skeletal muscle tissue. 293T cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Alternative versions available:

    Anti-Acetyl Coenzyme A carboxylase alpha antibody (Alexa Fluor® 488) [EPR4971] (ab203994)

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Applications

Our Abpromise guarantee covers the use of ab109368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 266 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionCatalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
    • Tissue specificityExpressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
    • PathwayLipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
    • Involvement in diseaseDefects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:200350]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.
    • Sequence similaritiesContains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • Post-translational
      modifications
      Phosphorylation on Ser-1263 is required for interaction with BRCA1.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAC antibody
      • ACACA antibody
      • ACACA_HUMAN antibody
      • ACC alpha antibody
      • ACC-alpha antibody
      • ACC1 antibody
      • ACCA antibody
      • Acetyl Coenzyme A antibody
      • Biotin carboxylase antibody
      see all

    Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] images

    • Overlay histogram showing SH-SY5Y cells stained with ab109368 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109368, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] (ab109368) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 266 kDa
    • Immunofluorescent staining of 293 cells using ab109368 at 1/100 dilution
    • Immunohistochemical analysis of paraffin-embedded skeletal muscle tissue using ab109368 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin-embedded brain tissue using ab109368 at 1/250 dilution.

    References for Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR4971] (ab109368)

    ab109368 has not yet been referenced specifically in any publications.

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