Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody (ab31931)
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Overview
- Product nameAnti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody
See all Acetyl Coenzyme A Carboxylase primary antibodies - DescriptionRabbit polyclonal to Acetyl Coenzyme A Carboxylase (phospho S79)
- SpecificityThis antibody recognizes Acetyl Coenzyme A Carboxylase phosphorylated at Ser79.
- Tested applicationsSuitable for: WBmore details
- Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Predicted to work with: Sheep, Goat, Chicken, Cow
- Immunogen
Synthetic peptide: C-HMRSSM[pS]GLHLVK conjugated to KLH, corresponding to amino acids 73-85 of Rat Acetyl Coenzyme A Carboxylase.
- Positive control
- RIPA lysates from mouse heart cytosol.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
- Storage bufferPreservative: 0.05% Sodium Azide.
Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4. -
Concentration information loading... - PurityProtein A purified
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
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Applications
Our Abpromise guarantee covers the use of ab31931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 265 kDa. Pretreating the blot with lambda phosphatase abolished antibody binding. |
Target
- FunctionCatalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
- Tissue specificityExpressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
- PathwayLipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
- Involvement in diseaseAcetyl-CoA carboxylase 1 deficiency
- Sequence similaritiesContains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain. - Post-translational
modificationsPhosphorylation on Ser-1263 is required for interaction with BRCA1. - Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 396504 Chicken
- Entrez Gene: 281590 Cow
- Entrez Gene: 31 Human
- Entrez Gene: 32 Human
- Entrez Gene: 100705 Mouse
- Entrez Gene: 107476 Mouse
- Entrez Gene: 116719 Rat
- Entrez Gene: 60581 Rat
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Alternative names
- ACAC antibody
- ACACA antibody
- ACACA_HUMAN antibody
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Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody images
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Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody (ab31931)
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 265 kDaMCF7 cells were incubated at 37°C for 6h with vehicle control (0 µM) and different concentrations of honokiol (ab120647). Increased expression of acetyl coenzyme A carboxylase (phospho S79) (ab31931) in MCF7 cells correlates with an increase in honokiol concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab31931 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
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Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody (ab31931)
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 265 kDaHepG2 cells were incubated at 37°C for 60 minutes with vehicle control (0 µM) and different concentrations of KU-55933 (ab120637). Decreased expression of Acetyl Coenzyme A Carboxylase (phospho S79) (ab31931) in HepG2 cells correlates with an increase in KU-55933 concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab31931 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
References for Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody (ab31931)
ab31931 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"