Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody (ab31931)

Overview

  • Product nameAnti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody
    See all Acetyl Coenzyme A Carboxylase primary antibodies
  • Description
    Rabbit polyclonal to Acetyl Coenzyme A Carboxylase (phospho S79)
  • SpecificityThis antibody recognizes Acetyl Coenzyme A Carboxylase phosphorylated at Ser79.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
    Predicted to work with: Sheep, Goat, Chicken, Cow
  • Immunogen

    Synthetic peptide: C-HMRSSM[pS]GLHLVK conjugated to KLH, corresponding to amino acids 73-85 of Rat Acetyl Coenzyme A Carboxylase.

  • Positive control
    • RIPA lysates from mouse heart cytosol.

Associated products

Applications

Our Abpromise guarantee covers the use of ab31931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 265 kDa. Pretreating the blot with lambda phosphatase abolished antibody binding.

Target

Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody images


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 265 kDa

    MCF7 cells were incubated at 37°C for 6h with vehicle control (0 µM) and different concentrations of honokiol (ab120647). Increased expression of acetyl coenzyme A carboxylase (phospho S79) (ab31931) in MCF7 cells correlates with an increase in honokiol concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab31931 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 265 kDa

    HepG2 cells were incubated at 37°C for 60 minutes with vehicle control (0 µM) and different concentrations of KU-55933 (ab120637). Decreased expression of Acetyl Coenzyme A Carboxylase (phospho S79) (ab31931) in HepG2 cells correlates with an increase in KU-55933 concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab31931 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

References for Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody (ab31931)

ab31931 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"