Recombinant
RabMAb

Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191)

Overview

  • Product name
    Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y]
    See all Acetyl Coenzyme A Carboxylase primary antibodies
  • Description
    Rabbit monoclonal [EP1885Y] to Acetyl Coenzyme A Carboxylase (phospho S79)
  • Specificity
    This antibody detects Acetyl Coenzyme A Carboxylase phosphorylated at serine 79.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Acetyl Coenzyme A Carboxylase (phospho S79). The exact sequence is proprietary.

  • Positive control
    • WB: A431 cell lysate, PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate (treated and untreated), RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate (treated and untreated).
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab68191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 265 kDa (predicted molecular weight: 277 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.
    • Tissue specificity
      Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.
    • Pathway
      Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.
    • Involvement in disease
      Acetyl-CoA carboxylase 1 deficiency
    • Sequence similarities
      Contains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.
    • Post-translational
      modifications
      Phosphorylation on Ser-1263 is required for interaction with BRCA1.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACAC antibody
      • ACACA antibody
      • ACACA_HUMAN antibody
      • ACACB antibody
      • ACC alpha antibody
      • ACC antibody
      • ACC beta antibody
      • ACC-alpha antibody
      • ACC1 antibody
      • ACC2 antibody
      • ACCA antibody
      • ACCB antibody
      • Acetyl CoA carboxylase 1 antibody
      • Acetyl CoA carboxylase 2 antibody
      • Acetyl CoA carboxylase alpha antibody
      • Acetyl CoA carboxylase beta antibody
      • Acetyl Coenzyme A carboxylase alpha antibody
      • Acetyl Coenzyme A carboxylase beta antibody
      • Biotin carboxylase antibody
      • COA1 antibody
      • COA2 antibody
      • HACC275 antibody
      • OTTHUMP00000164069 antibody
      • OTTHUMP00000164070 antibody
      • OTTHUMP00000164076 antibody
      • OTTHUMP00000240532 antibody
      see all

    Images

    • All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/1000 dilution

      Lane 1 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate. Then the membrane was incubated with phosphatase.
      Lane 2 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate.

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 277 kDa
      Observed band size : 265 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 seconds

      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/1000 dilution

      Lane 1 : RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate. Then the membrane was incubated with phosphatase.
      Lane 2 : Untreated RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate.

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 277 kDa
      Observed band size : 265 kDa (why is the actual band size different from the predicted?)


      Exposure time : 8 seconds

      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/10000 dilution

      Lane 1 : Untreated A431 whole cell lysate
      Lane 2 : A431 whole cell lysate - treated with lambda phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/2000 dilution

      Predicted band size : 277 kDa
      Observed band size : 265 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot blot analysis of Acetyl Coenzyme A Carboxylase (pS79) phospho peptide (lane 1) and Acetyl Coenzyme A Carboxylase non-phospho peptide (lane 2) labelling Acetyl Coenzyme A Carboxylase (phospho S79) with ab68191 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    References

    This product has been referenced in:
    • Meng X  et al. Liver MicroRNA-291b-3p Promotes Hepatic Lipogenesis through Negative Regulation of Adenosine 5'-Monophosphate (AMP)-activated Protein Kinase a1. J Biol Chem 291:10625-34 (2016). Read more (PubMed: 27013659) »
    • Theodoropoulou S  et al. Aminoimidazole carboxamide ribonucleotide (AICAR) inhibits the growth of retinoblastoma in vivo by decreasing angiogenesis and inducing apoptosis. PLoS One 8:e52852 (2013). WB ; Human . Read more (PubMed: 23300996) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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