Anti-acetyl Lysine antibody [1C6] - ChIP Grade (ab22550)

Overview

  • Product nameAnti-acetyl Lysine antibody [1C6] - ChIP GradeSee all acetyl Lysine primary antibodies ...
  • Description
    Mouse monoclonal [1C6] to acetyl Lysine - ChIP Grade
  • Specificityab22550 recognises proteins with acetylated lysine.
  • Tested applicationsIP, ChIP, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Non Human Primates
  • Immunogen

    Synthetic peptide: sequence surrounding the acetylated lysine 9 of histone H3

  • Positive control
    • HeLa cell lysate. MCF7 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab22550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 3 µg/ml.
ChIP Use a concentration of 10 µg/ml.
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/2000.

Target

  • RelevanceIn the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.
  • Alternative names
    • acetyl Lysine antibody
    • Lysine antibody

Anti-acetyl Lysine antibody [1C6] - ChIP Grade images

  • ICC/IF image of ab22550 stained MCF7 cells. The cells were 4% formaldehye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22550, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-acetyl Lysine antibody [1C6] - ChIP Grade (ab22550)

This product has been referenced in:
  • Stice JP  et al. 17{beta}-Estradiol, Aging, Inflammation, and the Stress Response in the Female Heart. Endocrinology 152:1589-98 (2011). WB ; Rat . Read more (PubMed: 21303943) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (15)
Sample Mouse Cell lysate - whole cell (Hepatocyte)
Specification Hepatocyte
Blocking step I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Mar 11 2014

Thank you very much for your interest in ab22550. To our knowledge, ab22550 has not been tested in IP. Therefore, I can offer a discount off a future purchase if you buy ab22550 now, test it in IPand submit feedback to us in the form of an Abreview. It...

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Application Western blot
Sample Human Cell lysate - whole cell (Jurkat cells)
Loading amount 10 µg
Specification Jurkat cells
Treatment trichostatin A 250 nM treated cells for 24h
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 19 2010

Thank you for your enquiry. The synthetic peptide used to raise this antibody was a peptide sequence surrounding the acetylated lysine 9 of histone H3. This antibody has been shown to generate a ladder in a HeLa whole cell lysate demonstrating that...

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Thank you for your enquiry. Yes, by Western blot, this antibody detects a ladder of acetylated proteins in HeLa cell lysates. In immunofluorescence procedures, this antibody recognizes acetylated lysine in gamma irradiated HeLa cells. I hope this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"