Overview

  • Product nameAnti-acetyl Lysine antibody
    See all acetyl Lysine primary antibodies
  • Description
    Sheep polyclonal to acetyl Lysine
  • SpecificityThe antibody has also been shown to react against histones, PCAF and E2F in Western blotting. Its suitability as a pan-specific anti-acetylated lysine antibody is currently under evaluation. Immunohistochemistry showed that the antiserum stains human and mouse chromosome preparations in a pattern that correlates with the acetylation as established by other means (unpublished data) and is equivalent to the rabbit polyclonal described in Turner & Fellows.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Tetra-acetylated synthetic N-terminal peptide, corresponding to amino acids 1-18 of Human histone H4.

  • Positive control
    • ICC/IF: HeLa cells gamma irradiated with 10-20 Gy
  • General notesThe product is filtered to 0.2 µm.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
  • Application notesELISA: Use at an assay dependent concentration.
    ICC/IF: 1/100. Fix cells with PFA.
    WB: Use at an assay dependent concentration.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceIn the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.
    • Alternative names
      • pan acetyl Lysine antibody

    References for Anti-acetyl Lysine antibody (ab76)

    This product has been referenced in:
    • Mohrmann L  et al. Differential targeting of two distinct SWI/SNF-related Drosophila chromatin-remodeling complexes. Mol Cell Biol 24:3077-88 (2004). ICC/IF . Read more (PubMed: 15060132) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. This antibody is currently under evaluation for its use as a pan-acetyl-lysine antibody. However, the immunogen used to raise this antibody was a tetra-acetylated synthetic N-terminal peptide, corresponding to amino acid...

    Read More

    Thank you for your enquiry. My colleague Dr Sarah Mardle is out of office until Thursday morning. As soon as she gets back, she will get in touch with you and deal with your enquiry. Thank you for your patience.

    We have not further tested the cross-reactivity of this antibody, all we can say is that it is likely to cross react with yeast based on sequence homology. I would expect it to work in yeast as there is a high level of histone homology between species.

    We do not currently have monoclonals to the different isoforms, but expect to add these to the range soon. Abcam

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"