Anti-acetyl Lysine antibody (Biotin) (ab26332)

Overview

  • Product nameAnti-acetyl Lysine antibody (Biotin)
    See all acetyl Lysine primary antibodies
  • Description
    Rabbit polyclonal to acetyl Lysine (Biotin)
  • ConjugationBiotin
  • Specificityab26332 detects proteins containing acetylated lysine residues. The product has been cross-adsorbed against the non-acetylated lysine to remove antibodies that may detect the non-acetylated form of the amino acid.
  • Tested applicationsSuitable for: WB, IP, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Acetylated KLH.

  • General notesStorage below -20°C is not recommended.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.1M PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was purified on an acetylated lysine bound immunoaffinity column.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab26332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
ELISA
  • Application notesELISA: Use at a concentration of 0.25 µg/ml.
    IF: 1/50.
    IP: Use 10µg per sample.
    WB: Use at a concentration of 1 µg/ml.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceIn the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.
    • Alternative names
      • pan acetyl Lysine antibody

    References for Anti-acetyl Lysine antibody (Biotin) (ab26332)

    ab26332 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"