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Acetylated KLH conjugates.
Use to detect acetylation of lysine.
Our Abpromise guarantee covers the use of ab21623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. PubMed: 24160175|
|ChIP||Use 2-4 µg for 25 µg of chromatin.|
|ICC/IF||1/100 - 1/250. Subconfluent MMRU cells seeded in a 6-well plate with or without HDAC inhibitor treatment were fixed and permeabilized with 1% formaldehyde and 0.5% Triton X-100 for 10 minutes and blocked with 10 mg mL-1 BSA for 1 hour at room temperature. Cells were stained with FITC-conjugated anti-acetylated lysine antibody at 1:100 dilution with PBSt 1 % BSA for 1 hour at room temperature.|
|WB||1/1000 - 1/2500.|
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 2µg of ab21623 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
Immunofluorescent staining of Human melanoma cells, using Rabbit polyclonal to acetyl Lysine (ab21623) at 1:100 dilution.
1: Untreated cells
2: TSA treated cells