HRP Anti-acetyl Lysine antibody (ab23364)
Key features and details
- HRP Rabbit polyclonal to acetyl Lysine
- Suitable for: ELISA, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
-
Product name
HRP Anti-acetyl Lysine antibody
See all acetyl Lysine primary antibodies -
Description
HRP Rabbit polyclonal to acetyl Lysine -
Host species
Rabbit -
Conjugation
HRP -
Specificity
This antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP, no reaction to the non-acetylated proteins. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Acetylated KLH conjugates.
-
General notes
The purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct label of primary anti-AcK will avoid the use of secondary antiboides therefore eliminating the interference of the 2nd antibody-conjugates.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6
Constituents: 0.268% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Primary antibody notes
The purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct label of primary anti-AcK will avoid the use of secondary antiboides therefore eliminating the interference of the 2nd antibody-conjugates. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent dilution.
Microarray: Use at an assay dependent dilution. |
|
WB |
Use at an assay dependent dilution. Detects a band of approximately 3 kDa.
|
Notes |
---|
ELISA
Use at an assay dependent dilution. Microarray: Use at an assay dependent dilution. |
WB
Use at an assay dependent dilution. Detects a band of approximately 3 kDa. |
Target
-
Relevance
In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo. -
Alternative names
- pan acetyl Lysine antibody
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab23364 has been referenced in 2 publications.
- Longo JF et al. ErbB4 promotes malignant peripheral nerve sheath tumor pathogenesis via Ras-independent mechanisms. Cell Commun Signal 17:74 (2019). PubMed: 31291965
- van Gent R et al. SIRT1 mediates FOXA2 breakdown by deacetylation in a nutrient-dependent manner. PLoS One 9:e98438 (2014). WB ; Human . PubMed: 24875183