Overview

  • Product nameAnti-acetyl Lysine antibody (HRP)
    See all acetyl Lysine primary antibodies
  • Description
    Rabbit polyclonal to acetyl Lysine (HRP)
  • ConjugationHRP
  • SpecificityThis antibody recognizes proteins acetylated on lysine residues. Tested: acetylated histone, acetylated BSA, and acetylated MBP, no reaction to the non-acetylated proteins.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Acetylated KLH conjugates.

  • General notes


    The purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct label of primary anti-AcK will avoid the use of secondary antiboides therefore eliminating the interference of the 2nd antibody-conjugates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 0.1M PBS, pH 7
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesThe purified antibody was conjugated to horse radish peroxidase (HRP) via reductive amination. Direct label of primary anti-AcK will avoid the use of secondary antiboides therefore eliminating the interference of the 2nd antibody-conjugates.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
Microarray: Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 3 kDa.

Target

  • RelevanceIn the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.
  • Alternative names
    • pan acetyl Lysine antibody

References for Anti-acetyl Lysine antibody (HRP) (ab23364)

This product has been referenced in:
  • van Gent R  et al. SIRT1 mediates FOXA2 breakdown by deacetylation in a nutrient-dependent manner. PLoS One 9:e98438 (2014). WB ; Human . Read more (PubMed: 24875183) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"