Recombinant

Anti-acetyl Lysine antibody [RM101] (ab190479)

Overview

  • Product name
    Anti-acetyl Lysine antibody [RM101]
    See all acetyl Lysine primary antibodies
  • Description
    Rabbit monoclonal [RM101] to acetyl Lysine
  • Host species
    Rabbit
  • Specificity
    ab190479 reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, or lysine with other modification.
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ChIP, Flow Cyt, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Synthetic peptide corresponding to acetyl Lysine conjugated to bovine serum albumin.

  • Positive control
    • A431 cells treated with Trichostatin A; HeLa whole cell lysate - Trichostatin A treated

Properties

Applications

Our Abpromise guarantee covers the use of ab190479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/500 - 1/2000.
IHC-P 1/100 - 1/500.
ChIP 1/100 - 1/500.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/100 - 1/500.
ICC/IF 1/100 - 1/500.

Target

  • Relevance
    In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.
  • Alternative names
    • pan acetyl Lysine antibody

Images

  • Lane 1: A431 whole cell lysate

    Lane 2: A431 whole cell lysate (pretreated with Trichostatin A)

    Lane 3: A431 whole cell lysate immunoprecipitated with Rabbit IgG

    Lane 4: A431 whole cell lysate (pretreated with Trichostatin A) immunoprecipitated with Rabbit IgG

    Lane 5: A431 whole cell lysate immunoprecipitated with ab190479 at 1/500
    Lane 6: A431 whole cell lysate (pretreated with Trichostatin A) immunoprecipitated with ab190479 at 1/500

     

    Western blot performed using anti-PTEN mouse monoclonal antibody.

  • Immunocytochemical staining of HeLa cells labelling Acetyl Lysine with ab190479 at 1:100. Actin filaments are labelled using fluorescein phalloidin (green), and nuclei are stained with DAPI (blue).

  • All lanes : Anti-acetyl Lysine antibody [RM101] (ab190479) at 1/2000 dilution

    Lanes 1 & 3 & 5 : Lysate of nontreated HeLa cells
    Lanes 2 & 4 & 6 : Lysate of HeLa cells treated with Trichostatin A

    Developed using the ECL technique.


    Exposure time increased from blot on left (lanes 1, 2) to blot on right (lanes 5,6).

  • Immunofluorescent analysis of A431cells nontreated (left) or treated with Trichostatin A (right), using ab190479 at 1/500 followed by a PE conjugated secondary antibody (red) and DAPI (blue).

References

This product has been referenced in:
  • Jin X  et al. TXNIP mediates the differential responses of A549 cells to sodium butyrate and sodium 4-phenylbutyrate treatment. Cancer Med : (2016). Read more (PubMed: 28033672) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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