Anti-Acetylated alpha Tubulin antibody [6-11B-1] (ab24610)

Overview

  • Product nameAnti-Acetylated alpha Tubulin antibody [6-11B-1]See all Acetylated alpha Tubulin primary antibodies ...
  • Description
    Mouse monoclonal [6-11B-1] to Acetylated alpha Tubulin
  • SpecificityThis antibody reacts to Acetylated a-Tubulin.
  • Tested applicationsFlow Cyt, IP, WB, ICC, IHC-P, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human, Sea Urchin
    Predicted to work with: Cow
  • Immunogen

    Tissue/ cell preparation - acetylated alpha-tubulin from the axoneme of sea urchin sperm flagella.

  • Positive control
    • In Western Blot, this antibody gave a positive signal in mouse brain tissue lysate and in the following whole cell lysates: HeLa; NIH3T3; PC12.
  • General notes


    This antibody binds to primary cilia, centrioles, mitotic spindles, midbodies and to subsets of cytoplasmic microtubules in 3T3 and HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.40
    Constituent: Ascites
  • Concentration information loading...
  • PurityAscites
  • Primary antibody notes This antibody binds to primary cilia, centrioles, mitotic spindles, midbodies and to subsets of cytoplasmic microtubules in 3T3 and HeLa cells.
  • Clonality Monoclonal
  • Clone number6-11B-1
  • IsotypeIgG2b
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab24610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µl for 106 cells.
IP Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC Use a concentration of 1 µg/ml.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/200 - 1/1000.
IHC-Fr 1/50.

Target

  • RelevanceMicrotubules are required for many well characterized functions in eukaryotic cells, including the movement of chromosomes in mitosis and meiosis, intracellular transport, establishment and maintenance of cellular morphology, cell growth, cell migration and morphogenesis in multicellular organisms. The building block of a microtubule is the tubulin subunit, a heterodimer of alpha and beta-tubulin. Both of these monomers are found in all eukaryotes, and their sequences are highly conserved. Acetylated alpha tubulin is present in various microtubule structures and plays a role in stabilizing the structures of all microtubules.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • alpha 1b antibody
    • Alpha tubulin 1 antibody
    • Alpha tubulin 2 antibody
    • Alpha-tubulin ubiquitous antibody
    • bA408E5.3 antibody
    • FLJ30169 antibody
    • H2 ALPHA antibody
    • K-ALPHA-1 antibody
    • Testis specific alpha tubulin antibody
    • TUBA 1 antibody
    • TUBA 2 antibody
    • TUBA1 antibody
    • TUBA2 antibody
    • Tubulin alpha 1 antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha 1 testis specific antibody
    • Tubulin alpha 2 antibody
    • Tubulin alpha 2 chain antibody
    • Tubulin alpha-1B chain antibody
    • Tubulin alpha-ubiquitous chain antibody
    • Tubulin H2 alpha antibody
    • Tubulin K-alpha-1 antibody
    see all

Anti-Acetylated alpha Tubulin antibody [6-11B-1] images

  • All lanes : Anti-Acetylated alpha Tubulin antibody [6-11B-1] (ab24610) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
    Lane 4 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 140 kDa,25 kDa,35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 150 seconds
  • ICC/IF image of ab24610 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab24610, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • ab24610 at 1/100 dilution staining acetylated alpha tubulinin in prostate carcinoma by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed, permeabilized in Triton X-100 prior to blocking in 1% serum for 1 hour at 27°C and then incubated with ab24610 for 12 hours at 4°C. Alexa fluor® 546 donkey polyclonal to mouse Ig, diluted 1/500, was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab24610 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24610, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab24610 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24610, 1µg/1x106 cells) for 30 min at 22ºC. (This data was generated from a purified version of the antibody. Some lots are produced as ascites fluid. We suggest 1µl/1x106 cells for ascites preparations). The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Acetylated alpha Tubulin antibody [6-11B-1] (ab24610)

This product has been referenced in:
  • Brockman-Schneider RA  et al. Effects of vitamin D on airway epithelial cell morphology and rhinovirus replication. PLoS One 9:e86755 (2014). IHC-P ; Human . Read more (PubMed: 24475177) »
  • Saraga M  et al. Mechanism of cystogenesis in nephrotic kidneys: a histopathological study. BMC Nephrol 15:3 (2014). IHC-P ; Human . Read more (PubMed: 24397250) »

See all 28 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Other - Protein G Dynabeads
Sample Human Cell lysate - other (H23)
Specification H23
Total protein in input 250 µg
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Submitted Dec 18 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (U87 human primary Glioblastoma)
Specification U87 human primary Glioblastoma
Permeabilization Yes - 0.3% triton X 100
Fixative Paraformaldehyde
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Submitted Nov 11 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Neuro2A, mouse neuroblastoma)
Specification Neuro2A, mouse neuroblastoma
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Nov 11 2013

The FACS data on the datasheet was from cells stained with a purified version of the antibody for which the concentration was known to be 0.4 mg/ml. The antibody is currently produced as ascites fluid. The amount of specific antibody in ascites is typi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 37°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Sample Sheep Tissue sections (Sheep Kidney)
Specification Sheep Kidney
Permeabilization Yes - PBS Tween
Fixative Paraformaldehyde
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Submitted Jul 09 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Gliobastoma cell line)
Specification Gliobastoma cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Mr. Wenson David Rajan

Verified customer

Submitted May 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted Apr 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (fibroblast (mouse))
Loading amount 50 µg
Specification fibroblast (mouse)
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Mar 18 2013


We would recommend using a pH6 sodium citrate retrieval.

Thank you for your enquiry.

I can confirm that ab24610 antibody is sold as ascites. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Anti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"