Anti-Acetylated Proteins antibody - ChIP Grade (ab193)

Overview

  • Product name
    Anti-Acetylated Proteins antibody - ChIP Grade
  • Description
    Rabbit polyclonal to Acetylated Proteins - ChIP Grade
  • Specificity
    The prime activity is against the tri and tetra acetylated forms of histone H4. It also reacts with epsilon acetylated lysines. It also reacts with other acetylated proteins: BSA, E2F, PCAF, Importin, GATA1, BRCA2 and Rb.
  • Tested applications
    Suitable for: WB, IP, ELISA, ChIPmore details
  • Immunogen

    Chemically acetylated human histone H4

  • Positive control
    • mammalian histones
  • General notes

    This antibody recognises a wide range of acetylated proteins.

Properties

Applications

Our Abpromise guarantee covers the use of ab193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
ChIP Use at an assay dependent dilution. Recommended to use 5-10µl for 25µg of chromatin.

Images

  • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 5µl of  ab193 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
        


  • Developed using the ECL technique

    Exposure time : 5 minutes

    Image courtesy of an Abreview submitted by J Stanley.

    ab193 used in Western Blotting.

    Lysate prepared from T47D cells was loaded at 100µg. Cells were pre-treated with either insulin, butyrate, resveratrol or trichostatin A. Primary antibody incubated for 24 hours at a 1/1000 dilution. An HRP-conjugated F(ab)2 secondary antibody was used at a 1/8000 dilution.

    Left arrows indicate signal increased by treatment with inhibitors of deacetylases. The nuclear protein increased by butyrate and trichostatin A is probably histone H4 based on its size. One unidentified protein of approximately 65kDa is increased by the treatments in cytoplasm.

References

This product has been referenced in:
  • Kessler R  et al. dDsk2 regulates H2Bub1 and RNA polymerase II pausing at dHP1c complex target genes. Nat Commun 6:7049 (2015). ChIP . Read more (PubMed: 25916810) »
  • Norouzitallab P  et al. Environmental heat stress induces epigenetic transgenerational inheritance of robustness in parthenogenetic Artemia model. FASEB J 28:3552-63 (2014). WB . Read more (PubMed: 24755740) »

See all 21 Publications for this product

Customer reviews and Q&As



Nous avons un anticorps "anti-Acetylated Proteins" testé en IP et Western Blot, le ab193. Le lien vers la fiche technique est http://www.abcam.com/ab193.

Thank you for your kind response. As I have explained in my previous e-mail any human lysate will probably have high levels of histone acetylation. However, it may be useful to treat the cells to maximize the level of acetylation. We have a very inform...

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Thank you for your response. First of all, I can offer a discount code for testing this antibody in untested species. Please take a look at this initiative and the conditions and let me know if you wish to proceed: www.abcam.com/collaborationdiscount....

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Thank you for your enquiry regarding ab193. I can confirm that this antibody detects any acetylated proteins and it should work in many mammalian species. Unfortunately, it has not been tested specifically in crustaceans at all. Theoretically, as long ...

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Thank you for contacting us. We do not currently sell an antibody to DAP1 that is specific for the N-acetylserine modification. If you are looking to detect this modification, you may be able to perform an IP for the total DAP1 protein using ab32056 ...

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Thank you for your enquiry. I can confirm that ab193 is raised against hyperacetylated histone H4 and indeed recognizes AcH4 very well. It also exhibits some activity against epsilon-acetyl lysine in some other proteins, such as Gata1 (see Hung...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (T47D)
Loading amount
100 µg
Specification
T47D
Treatment
insulin, butyrate, resveratrol, TSA
Blocking step
Serum, BSA, milk sequential incubations as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. F Stanley

Verified customer

Submitted Mar 06 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 colon cancer cell line)
Loading amount
40 µg
Specification
HCT116 colon cancer cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% in TBST
Username

Abcam user community

Verified customer

Submitted Sep 18 2006

Thank you for your enquiry. Rabbit polyclonal to Acetylated Proteins - ChIP Grade (ab193) was raised using an immunogen of chemically acetylated human histone H4. It does not carry specificity for a particular acetyl-lysine residue. The reactivity ...

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Thank you for your enquiry. The immunogen was chemically acetylated histone H4 (H4 treated with acetic anhydride) and it works well in enriching chromatin (ChIPs) in almost all acetylated forms of H4 (though we have not tried it specifically with s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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