Anti-Acetylcholinesterase antibody [4E11] (ab17774)

Overview

  • Product name
    Anti-Acetylcholinesterase antibody [4E11]
    See all Acetylcholinesterase primary antibodies
  • Description
    Mouse monoclonal [4E11] to Acetylcholinesterase
  • Specificity
    ab17774 is specific for brain AChE and does not recognize AChE from erythrocytes.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Cow, Human
    Predicted to work with: Rabbit, Cat
  • Immunogen

    Synthetic peptide corresponding to Human Acetylcholinesterase aa 574-583.
    Sequence:

    TDTLDEA ERQ


    Database link: P22303

  • Epitope
    C-terminal 10 residues (aa 574-583) of human brain acetylcholinesterase.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purification notes
    Protein A/G purified.
  • Clonality
    Monoclonal
  • Clone number
    4E11
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/200. Predicted molecular weight: 68 kDa.

Target

  • Function
    Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
  • Tissue specificity
    Isoform H is highly expressed in erythrocytes.
  • Sequence similarities
    Belongs to the type-B carboxylesterase/lipase family.
  • Cellular localization
    Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACEE antibody
    • ACES_HUMAN antibody
    • Acetylcholinesterase antibody
    • AChE antibody
    • Apoptosis related acetylcholinesterase antibody
    • ARACHE antibody
    • N ACHE antibody
    • N-ACHE antibody
    • YT antibody
    • YT blood group antibody
    see all

References for Anti-Acetylcholinesterase antibody [4E11] (ab17774)

This product has been referenced in:
  • Boschetti N  et al. Monoclonal antibodies against a C-terminal peptide of human brain acetylcholinesterase distinguish between erythrocyte and brain acetylcholinesterases. Clin Chem 42:19-23 (1996). Read more (PubMed: 8565226) »

See 1 Publication for this product

Product Wall

Merci de nous avoir contactés. Ma collègue m'a expliqué les raisons de votre appel.

1) ab17774: nos produits sont garantis pour fonctionner tels qu'ils sont décrits sur la fiche technique (si le problème nous est communiqué dans les 6 mois qui...

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Thank you for your enquiry. I have taken a closer look at the article the original developers of the antibody published (Boschetti et al. Clinical Chemistry 42:1, 19-23 (1996)). It seems they are using a 5 ug/ml dilution in their Western blotting ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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