Anti-Acetylcholinesterase antibody (ab31276)
Key features and details
- Goat polyclonal to Acetylcholinesterase
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Acetylcholinesterase antibody
See all Acetylcholinesterase primary antibodies -
Description
Goat polyclonal to Acetylcholinesterase -
Host species
Goat -
Specificity
This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form). -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Cow, Cat -
Immunogen
Synthetic peptide:
QFDHYSKQDRCSDL
, corresponding to C terminal amino acids 601-614 of Human Acetylcholinesterase -
Positive control
- WB: Jurkat, HepG2, SkHep1, Snu387, and SW620 cell lysates. Flow Cyt: HeLa cells ICC/IF: U2OS cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (3) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
1 hour primary incubation is recommended for this product. |
ICC/IF |
Use a concentration of 10 µg/ml.
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|
Flow Cyt |
Use a concentration of 10 µg/ml.
|
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
Flow Cyt
Use a concentration of 10 µg/ml. |
Target
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Function
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. -
Tissue specificity
Isoform H is highly expressed in erythrocytes. -
Sequence similarities
Belongs to the type-B carboxylesterase/lipase family. -
Cellular localization
Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei. - Information by UniProt
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Database links
- Entrez Gene: 540446 Cow
- Entrez Gene: 43 Human
- Entrez Gene: 83817 Rat
- Omim: 100740 Human
- SwissProt: P23795 Cow
- SwissProt: P22303 Human
- SwissProt: Q29499 Rabbit
- SwissProt: P37136 Rat
see all -
Alternative names
- ACEE antibody
- ACES_HUMAN antibody
- Acetylcholinesterase antibody
see all
Images
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Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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All lanes : Anti-Acetylcholinesterase antibody (ab31276) at 0.3 µg/ml
Lane 1 : Jurkat cell lysate at 0.5 µg/ml
Lane 2 : HepG2 cell lysate in RIPA buffer at 35 µg
Predicted band size: 68 kDaDetected by chemiluminescence.
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Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing nuclear, membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
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All lanes : Anti-Acetylcholinesterase antibody (ab31276) at 1/200 dilution
Lane 1 : SkHep1 cell lysate
Lane 2 : Snu387 cell lysate
Lane 3 : SW620 cell lysate
Lane 4 : Zebrafish brain lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated Rabbit anti-goat IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Exposure time: 20 minutes
Datasheets and documents
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Datasheet download
References (13)
ab31276 has been referenced in 13 publications.
- Martin-Jaular L et al. Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection. EMBO J 40:e105492 (2021). PubMed: 33709510
- Atali S et al. Interaction of amyloid beta with humanin and acetylcholinesterase is modulated by ATP. FEBS Open Bio 10:2805-2823 (2020). PubMed: 33145964
- Cocozza F et al. Extracellular vesicles containing ACE2 efficiently prevent infection by SARS-CoV-2 Spike protein-containing virus. J Extracell Vesicles 10:e12050 (2020). PubMed: 33391636
- Yekta R et al. The inefficacy of donepezil on glycated-AChE inhibition: Binding affinity, complex stability and mechanism. Int J Biol Macromol 160:35-46 (2020). PubMed: 32454110
- Price D et al. Humanin Blocks the Aggregation of Amyloid-ß Induced by Acetylcholinesterase, an Effect Abolished in the Presence of IGFBP-3. Biochemistry 59:1981-2002 (2020). PubMed: 32383868