Anti-Acetylcholinesterase antibody (ab31276)

Overview

  • Product name
    Anti-Acetylcholinesterase antibody
    See all Acetylcholinesterase primary antibodies
  • Description
    Goat polyclonal to Acetylcholinesterase
  • Specificity
    This antibody is expected to recognise isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Cow, Cat
  • Immunogen

    Synthetic peptide:

    QFDHYSKQDRCSDL

    , corresponding to C terminal amino acids 601-614 of Human Acetylcholinesterase

  • Positive control
    • Human fibroblast cell lines A431 and HeLa lysates. Human cerebellum lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).

Target

Anti-Acetylcholinesterase antibody images

  • All lanes : Anti-Acetylcholinesterase antibody (ab31276) at 1/200 dilution

    Lane 1 : SkHep1 cell lysate
    Lane 2 : Snu387 cell lysate
    Lane 3 : SW620 cell lysate
    Lane 4 : Zebrafish brain lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Rabbit anti-goat IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa


    Exposure time : 20 minutes

    This image is courtesy of an Abreview submitted by Ender Avci

    See Abreview

  • Anti-Acetylcholinesterase antibody (ab31276) at 0.1 µg/ml + Human Fibroblast A431 lysate (35µg protein in RIPA buffer)

    Predicted band size : 68 kDa
    Observed band size : 60-70 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Anti-Acetylcholinesterase antibody (ab31276) at 1 µg/ml + human cerebellum lysate (35µg protein in RIPA buffer).

    Predicted band size : 68 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour.
    Detected by chemiluminescence.

References for Anti-Acetylcholinesterase antibody (ab31276)

This product has been referenced in:
  • Vetreno RP  et al. Adolescent, but not adult, binge ethanol exposure leads to persistent global reductions of choline acetyltransferase expressing neurons in brain. PLoS One 9:e113421 (2014). WB ; Human . Read more (PubMed: 25405505) »
  • García-Ayllón MS  et al. Readthrough acetylcholinesterase is increased in human liver cirrhosis. PLoS One 7:e44598 (2012). WB ; Human . Read more (PubMed: 23028565) »

See all 5 Publications for this product

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (4-20%)
Sample
Mouse Tissue lysate - whole (Hippocampus)
Specification
Hippocampus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Sep 02 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Zebrafish Tissue lysate - other (zebrafish brain)
Specification
zebrafish brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ender Avci

Verified customer

Submitted Jul 08 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
ELISA
Sample
Human Purified protein (human acetylcholinesterase from erythrocytes)
Specification
human acetylcholinesterase from erythrocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type
Direct
Username

Abcam user community

Verified customer

Submitted Apr 29 2013

Thank you for your email.

I have done a homology search between human ACHE and Danio Rerio (Zebrafish). The data shows intermittent homology, please check

http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2...

Read More

Thank you for your enquiry.

I am sorry to confirm I am unable to find the cDNA sequence of acetylcholinesterase isoform T. We do have a link to the Unigene protein database site which has information regarding isoform T:

http://ww...

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Thank you for your enquiry. I have enquired at the originator of the product and according to their immunizing peptide sequence blast result, there is no match with butyrylcholinesterase. Therefore, this product should not cross react with butyrlch...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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