Anti-Acetylcholinesterase antibody [AE-2] (ab77959)

Overview

  • Product nameAnti-Acetylcholinesterase antibody [AE-2]See all Acetylcholinesterase primary antibodies ...
  • Description
    Mouse monoclonal [AE-2] to Acetylcholinesterase
  • Tested applicationsFlow Cyt, WB, IP, IHC-FoFr more details
  • Species reactivity
    Reacts with: Rat, Human, Monkey
  • Immunogen

    Full length native Acetylcholinesterase protein (purified from Human erythrocyte)

  • Positive control
    • SKBR-3 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: Ascites, 0.25M Sodium chloride, 0.02M PBS, pH 7.2
  • Concentration information loading...
  • PurityAscites
  • Clonality Monoclonal
  • Clone numberAE-2
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab77959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 68 kDa.
IP Use at an assay dependent dilution.
IHC-FoFr 1/200 - 1/1000. Does not react with fixed tissue; add antibody to perfused but unfixed tissue, then fix after primary incubation.

Target

  • FunctionTerminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
  • Tissue specificityIsoform H is highly expressed in erythrocytes.
  • Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
  • Cellular localizationCell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
  • Target information above from: UniProt accession P22303 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • ACEE antibody
    • ACES_HUMAN antibody
    • Acetylcholine acetylhydrolase antibody
    • Acetylcholinesterase antibody
    • Acetylcholinesterase YT blood group antibody
    • ACHE antibody
    • ACHE protein antibody
    • Apoptosis related acetylcholinesterase antibody
    • ARACHE antibody
    • N ACHE antibody
    • N-ACHE antibody
    • YT antibody
    • Yt blood group antibody
    see all

Anti-Acetylcholinesterase antibody [AE-2] images

  • Overlay histogram showing HeLa cells stained with ab77959 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77959, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Acetylcholinesterase antibody [AE-2] (ab77959)

ab77959 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (retina)
Specification retina
Fixative Paraformaldehyde
Permeabilization Yes - triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ronit Heinrich

Verified customer

Submitted Aug 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"