The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesDot: Use at an assay dependent dilution.
ELISA: 1/1000 - 1/2000.
This product has been assayed against 1.0µg of Acetylcholinesterase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic-acid]) as a substrate for 30 minutes at RT.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 68 kDa.
Suitable for other antibody based assays using streptavidin or avidin comjugates.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTerminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
Tissue specificityIsoform H is highly expressed in erythrocytes.
Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
Cellular localizationCell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.