Anti-Acetylcholinesterase antibody (Biotin) (ab34533)

Overview

  • Product nameAnti-Acetylcholinesterase antibody (Biotin)
    See all Acetylcholinesterase primary antibodies
  • Description
    Goat polyclonal to Acetylcholinesterase (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, ELISA, Immunomicroscopy, Dot blotmore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    Acetylcholinesterase from Bovine Erythrocytes.

  • General notes

    10-20 Biotin molecules per goat IgG molecule.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and Protease free
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product is purified by a multi-step process including delipidation, salt fractionation, ion exchange chromatography and extensive dialysis against buffer.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Immunomicroscopy
Dot blot
  • Application notesDot: Use at an assay dependent dilution.
    ELISA: 1/1000 - 1/2000.
    This product has been assayed against 1.0µg of Acetylcholinesterase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic-acid]) as a substrate for 30 minutes at RT.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 68 kDa.
    Suitable for other antibody based assays using streptavidin or avidin comjugates.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionTerminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
    • Tissue specificityIsoform H is highly expressed in erythrocytes.
    • Sequence similaritiesBelongs to the type-B carboxylesterase/lipase family.
    • Cellular localizationCell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACEE antibody
      • ACES_HUMAN antibody
      • Acetylcholinesterase antibody
      • AChE antibody
      • Apoptosis related acetylcholinesterase antibody
      • ARACHE antibody
      • N ACHE antibody
      • N-ACHE antibody
      • YT antibody
      • YT blood group antibody
      see all

    References for Anti-Acetylcholinesterase antibody (Biotin) (ab34533)

    ab34533 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab34533.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"