Overview

  • Product name
    Anti-Acetylcholinesterase antibody (Biotin)
    See all Acetylcholinesterase primary antibodies
  • Description
    Goat polyclonal to Acetylcholinesterase (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: WB, ELISA, Immunomicroscopy, Dot blotmore details
  • Species reactivity
    Reacts with: Cow
  • Immunogen

    Acetylcholinesterase from Bovine Erythrocytes.

  • General notes

    10-20 Biotin molecules per goat IgG molecule.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA

    BSA is IgG and Protease free
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is purified by a multi-step process including delipidation, salt fractionation, ion exchange chromatography and extensive dialysis against buffer.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Immunomicroscopy
Dot blot
  • Application notes
    Dot: Use at an assay dependent dilution.
    ELISA: 1/1000 - 1/2000.
    This product has been assayed against 1.0µg of Acetylcholinesterase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic-acid]) as a substrate for 30 minutes at RT.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 68 kDa.
    Suitable for other antibody based assays using streptavidin or avidin comjugates.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.
    • Tissue specificity
      Isoform H is highly expressed in erythrocytes.
    • Sequence similarities
      Belongs to the type-B carboxylesterase/lipase family.
    • Cellular localization
      Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei.
    • Information by UniProt
    • Database links
    • Alternative names
      • ACEE antibody
      • ACES_HUMAN antibody
      • Acetylcholinesterase antibody
      • AChE antibody
      • Apoptosis related acetylcholinesterase antibody
      • ARACHE antibody
      • N ACHE antibody
      • N-ACHE antibody
      • YT antibody
      • YT blood group antibody
      see all

    References

    This product has been referenced in:
    • Freitas Leal JK  et al. Acetylcholinesterase provides new insights into red blood cell ageing in vivo and in vitro. Blood Transfus 15:232-238 (2017). Read more (PubMed: 28518050) »

    See 1 Publication for this product

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    There are currently no Customer reviews or Questions for ab34533.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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